Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11454 and 11455 are separated by 596 nucleotides11455 and 11456 are separated by 192 nucleotides11456 and 11457 overlap by 8 nucleotides11457 and 11458 are separated by 498 nucleotides mRNA_3086: 11454 - K03136 TFIIE1, GTF2E1, TFA1, tfe transcription initiation factor TFIIE subunit alpha, at 286,949 to 288,955 11454 mRNA_3087: 11455 - HMMPfam-haloacid dehalogenase-like hydrolase-PF12710,SUPERFAMILY--SSF56784,TIGRFAM-HAD-SF-IB HAD phosphoserine phosphatase-like hydrolase, family IB-TIGR01488,TIGRFAM-DKMTPPase-SF 2,3-diketo-5-methylthio-1-phosphopentane phosphatase-TIGR01489, at 289,552 to 290,619 11455 mRNA_3088: 11456 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 290,812 to 293,862 11456 mRNA_3089: 11457 - BLAST Rho GTPase-activating protein 30 [Larimichthys crocea], at 293,855 to 294,427 11457 mRNA_3090: 11458 - K03062 PSMC1, RPT2 26S proteasome regulatory subunit T2, at 294,926 to 296,826 11458
Group Condition mRNA_3086 mRNA_3087 mRNA_3088 mRNA_3089 mRNA_3090
11454 11455 11456 11457 11458
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 +0.0 -0.8 -1.7 +0.1
growth on ypd YPD +0.1 -0.2 -0.3 -0.3 -0.4
facs on lipid accumulation Sorted High BODIPY Gate -0.5 -0.4 -0.1 -0.1 +0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.3 -0.6 +0.0 -0.0 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 -0.2 -0.2 -0.1 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 -0.3 -0.3 -0.4 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 +0.1 -0.1 -0.6 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 -0.4 -0.3 +0.3 +0.0
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 -0.6 +0.2 +0.1 +0.1
auxotrophy YNB Glucose 75mM Methionine -0.4 -0.0 +0.1 -0.0 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 -0.2 +0.1 +0.2 -0.3
auxotrophy YNB Glucose 75mM Arginine -0.2 -0.1 -0.0 +0.0 +0.3
ypd buoyancy 40 hrs Low Buoyancy -0.3 -0.0 -0.1 +0.3 +0.2
growth on oleic acid YNB Glucose +0.1 -0.0 -0.1 +0.2 -0.1
growth on ricinoleic acid YNB Glucose -0.2 +0.1 +0.1 +0.0 +0.1
ypd buoyancy 40 hrs High Buoyancy -0.1 -0.0 +0.2 +0.5 -0.0
ypd buoyancy 40 hrs Median Buoyancy -0.4 +0.2 -0.1 +0.5 +0.3
growth on oleic acid YNB Oleic Acid -0.0 +0.1 +0.0 +0.1 +0.4
auxotrophy YNB Glucose -0.3 +0.2 +0.1 +0.0 +0.5
auxotrophy Dropout Complete -0.4 +0.2 +0.2 +0.4 +0.4
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 +0.3 +0.1 +0.5 -0.0
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