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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
11455 and 11456 are separated by 192 nucleotides
11456 and 11457 overlap by 8 nucleotides
11457 and 11458 are separated by 498 nucleotides
11458 and 11459 overlap by 84 nucleotides
mRNA_3087: 11455 - HMMPfam-haloacid dehalogenase-like hydrolase-PF12710,SUPERFAMILY--SSF56784,TIGRFAM-HAD-SF-IB HAD phosphoserine phosphatase-like hydrolase, family IB-TIGR01488,TIGRFAM-DKMTPPase-SF 2,3-diketo-5-methylthio-1-phosphopentane phosphatase-TIGR01489, at 289,552 to 290,619
11455
mRNA_3088: 11456 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 290,812 to 293,862
11456
mRNA_3089: 11457 - BLAST Rho GTPase-activating protein 30 [Larimichthys crocea], at 293,855 to 294,427
11457
mRNA_3090: 11458 - K03062 PSMC1, RPT2 26S proteasome regulatory subunit T2, at 294,926 to 296,826
11458
mRNA_3091: 11459 - K13303 SGK2 serum/glucocorticoid-regulated kinase 2, at 296,743 to 299,433
11459
Group
Condition
mRNA
_3087
mRNA
_3088
mRNA
_3089
mRNA
_3090
mRNA
_3091
11455
11456
11457
11458
11459
growth on ricinoleic acid
YNB Ricinoleic Acid
+0.0
-0.8
-1.7
+0.1
+0.1
growth on ypd
YPD
-0.2
-0.3
-0.3
-0.4
-0.1
facs on lipid accumulation
Sorted No BODIPY Gate
-0.6
+0.0
-0.0
+0.1
-0.3
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.3
-0.3
-0.4
-0.1
+0.3
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.2
-0.2
-0.1
-0.1
+0.2
facs on lipid accumulation
Sorted High BODIPY Gate
-0.4
-0.1
-0.1
+0.2
-0.0
growth on ricinoleic acid
YNB Methylricinoleic Acid
+0.1
-0.1
-0.6
+0.1
+0.1
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.6
+0.2
+0.1
+0.1
-0.1
growth on oleic acid
YNB Glucose
-0.0
-0.1
+0.2
-0.1
-0.0
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-0.4
-0.3
+0.3
+0.0
+0.5
buoyancy on lipid accumulation
40 hrs Low Buoyancy
-0.2
+0.1
+0.2
-0.3
+0.4
auxotrophy
YNB Glucose 75mM Arginine
-0.1
-0.0
+0.0
+0.3
+0.3
ypd buoyancy
40 hrs Low Buoyancy
-0.0
-0.1
+0.3
+0.2
+0.1
growth on ricinoleic acid
YNB Glucose
+0.1
+0.1
+0.0
+0.1
+0.2
auxotrophy
YNB Glucose 75mM Methionine
-0.0
+0.1
-0.0
+0.1
+0.4
ypd buoyancy
40 hrs High Buoyancy
-0.0
+0.2
+0.5
-0.0
-0.0
ypd buoyancy
40 hrs Median Buoyancy
+0.2
-0.1
+0.5
+0.3
-0.2
growth on oleic acid
YNB Oleic Acid
+0.1
+0.0
+0.1
+0.4
+0.1
ypd buoyancy
40 hrs No Buoyancy Separation
+0.3
+0.1
+0.5
-0.0
-0.1
auxotrophy
YNB Glucose
+0.2
+0.1
+0.0
+0.5
+0.3
auxotrophy
Dropout Complete
+0.2
+0.2
+0.4
+0.4
+0.3
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