Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11477 and 11478 are separated by 153 nucleotides11478 and 11479 are separated by 276 nucleotides11479 and 11480 are separated by 179 nucleotides11480 and 11481 are separated by 383 nucleotides mRNA_3109: 11477 - K13172 SRRM2, SRM300 serine/arginine repetitive matrix protein 2, at 344,614 to 345,945 11477 mRNA_3110: 11478 - K14558 PWP2, UTP1 periodic tryptophan protein 2, at 346,099 to 349,205 11478 mRNA_3111: 11479 - K09788 prpF 2-methylaconitate isomerase, at 349,482 to 350,899 11479 mRNA_3112: 11480 - Hypothetical Protein, at 351,079 to 352,331 11480 mRNA_3113: 11481 - K11361 SPT20, ADA5 transcription factor SPT20, at 352,715 to 353,874 11481
Group Condition mRNA_3109 mRNA_3110 mRNA_3111 mRNA_3112 mRNA_3113
11477 11478 11479 11480 11481
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 -0.1 -0.4 -0.1 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 -0.3 -0.1 -0.1 -0.4
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.3 -0.1 -0.1 +0.0
ypd buoyancy 40 hrs Median Buoyancy -0.1 -0.1 -0.1 -0.2 -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.1 -0.0 -0.1 -0.2 -0.2
ypd buoyancy 40 hrs High Buoyancy -0.1 -0.0 -0.0 -0.2 +0.0
facs on lipid accumulation Sorted High BODIPY Gate +0.1 -0.1 +0.0 -0.1 -0.2
growth on oleic acid YNB Oleic Acid -0.0 -0.0 +0.1 -0.1 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.4 -0.2 -0.3 +0.0 -0.2
growth on oleic acid YNB Glucose +0.1 -0.2 +0.1 -0.1 -0.0
growth on ypd YPD +0.4 +0.1 +0.1 -0.3 -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 -0.0 -0.1 -0.1 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 -0.2 -0.2 +0.1 +0.2
auxotrophy YNB Glucose 75mM Arginine -0.0 -0.0 +0.1 +0.6 -0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.4 -0.1 -0.1 -0.0 +0.0
auxotrophy YNB Glucose +0.1 -0.0 -0.1 +0.5 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 +0.0 -0.1 +0.1 +0.4
growth on ricinoleic acid YNB Glucose +0.4 -0.2 +0.2 +0.1 +0.0
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.0 +0.1 +0.5 -0.2
auxotrophy Dropout Complete +0.2 +0.0 +0.2 +0.5 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 -0.0 +0.2 +0.4 +0.2
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