Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11493 and 11494 are separated by 1176 nucleotides11494 and 11495 are separated by 27 nucleotides11495 and 11496 are separated by 89 nucleotides11496 and 11497 are separated by 227 nucleotides mRNA_3125: 11493 - K08145 SLC2A8, GLUT8 MFS transporter, SP family, solute carrier family 2 (facilitated glucose transporter), member 8, at 377,288 to 379,485 11493 mRNA_3126: 11494 - KOG0019 Molecular chaperone (HSP90 family), at 380,662 to 381,306 11494 mRNA_3127: 11495 - K11984 SART1, HAF, SNU66 U4/U6.U5 tri-snRNP-associated protein 1, at 381,334 to 384,253 11495 mRNA_3128: 11496 - BLAST protein transporter [Pseudozyma hubeiensis SY62], at 384,343 to 385,202 11496 mRNA_3129: 11497 - KOG4090 Uncharacterized conserved protein, at 385,430 to 386,354 11497
Group Condition mRNA_3125 mRNA_3126 mRNA_3127 mRNA_3128 mRNA_3129
11493 11494 11495 11496 11497
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 -0.3 -0.2 -0.2 -0.5
facs on lipid accumulation Sorted High BODIPY Gate -0.4 -0.1 +0.1 -0.1 -0.5
facs on lipid accumulation Sorted No BODIPY Gate +0.1 -0.1 -0.2 -0.2 -0.3
growth on oleic acid YNB Oleic Acid -0.2 -0.2 -0.1 -0.1 -0.0
auxotrophy YNB Glucose 75mM Arginine -0.2 -0.1 -0.2 -0.3 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 -0.5 -0.0 +0.4 -0.0
auxotrophy YNB Glucose 75mM Methionine -0.1 -0.1 +0.0 -0.2 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 -0.2 +0.0 +0.0 -0.2
growth on oleic acid YNB Glucose +0.2 -0.1 -0.1 -0.2 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.0 -0.0 -0.1 +0.1 -0.1
auxotrophy Dropout Complete +0.1 +0.0 -0.1 -0.1 -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.1 -0.2 +0.2 -0.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 -0.1 -0.2 +0.0 -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.3 -0.1 -0.1 +0.1 -0.2
auxotrophy YNB Glucose +0.1 -0.1 -0.1 -0.1 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.6 -0.1 +0.0 -0.1 -0.3
growth on ricinoleic acid YNB Glucose +0.3 -0.1 -0.1 -0.1 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 -0.0 -0.0 +0.4 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 -0.2 +0.0 +0.3 +0.0
growth on ypd YPD -0.3 +0.3 +0.1 +0.2 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.7 -0.0 -0.1 +0.3 +0.0
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