Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11541 and 11542 are separated by 481 nucleotides11542 and 11543 are separated by 446 nucleotides11543 and 11544 are separated by 369 nucleotides11544 and 11545 are separated by 277 nucleotides mRNA_3173: 11541 - K09497 CCT5 T-complex protein 1 subunit epsilon, at 7,947 to 10,272 11541 mRNA_3174: 11542 - K00953 FLAD1 FAD synthetase, at 10,754 to 11,960 11542 mRNA_3175: 11543 - K01191 E3.2.1.24 alpha-mannosidase, at 12,407 to 17,406 11543 mRNA_3176: 11544 - K17301 COPB1, SEC26 coatomer subunit beta, at 17,776 to 21,807 11544 mRNA_3177: 11545 - KOG2164 Predicted E3 ubiquitin ligase, at 22,085 to 24,622 11545
Group Condition mRNA_3173 mRNA_3174 mRNA_3175 mRNA_3176 mRNA_3177
11541 11542 11543 11544 11545
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +0.1 N.D. -0.7
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.1 N.D. -0.4
auxotrophy YNB Glucose N.D. N.D. -0.2 N.D. -0.2
growth on ypd YPD N.D. N.D. -0.1 N.D. -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +0.1 N.D. -0.5
auxotrophy Dropout Complete N.D. N.D. -0.2 N.D. -0.2
growth on oleic acid YNB Glucose N.D. N.D. -0.0 N.D. -0.4
growth on oleic acid YNB Oleic Acid N.D. N.D. +0.2 N.D. -0.4
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.1 N.D. -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.0 N.D. -0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +0.2 N.D. -0.3
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.1 N.D. +0.0
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.1 N.D. +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.1 N.D. -0.2
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.1 N.D. -0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. +0.1 N.D. +0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.0 N.D. +0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. +0.3 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.1 N.D. +0.0
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.1 N.D. +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.3 N.D. +0.1
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