Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11542 and 11543 are separated by 446 nucleotides11543 and 11544 are separated by 369 nucleotides11544 and 11545 are separated by 277 nucleotides11545 and 11546 are separated by 468 nucleotides mRNA_3174: 11542 - K00953 FLAD1 FAD synthetase, at 10,754 to 11,960 11542 mRNA_3175: 11543 - K01191 E3.2.1.24 alpha-mannosidase, at 12,407 to 17,406 11543 mRNA_3176: 11544 - K17301 COPB1, SEC26 coatomer subunit beta, at 17,776 to 21,807 11544 mRNA_3177: 11545 - KOG2164 Predicted E3 ubiquitin ligase, at 22,085 to 24,622 11545 mRNA_3178: 11546 - KOG4561 Uncharacterized conserved protein, contains TBC domain, at 25,091 to 26,696 11546
Group Condition mRNA_3174 mRNA_3175 mRNA_3176 mRNA_3177 mRNA_3178
11542 11543 11544 11545 11546
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.2 N.D. -0.3 -0.3
growth on oleic acid YNB Glucose N.D. -0.0 N.D. -0.4 +0.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.3 N.D. -0.1 -0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.3 N.D. +0.1 -0.7
auxotrophy Dropout Complete N.D. -0.2 N.D. -0.2 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.1 N.D. -0.7 +0.3
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.0 N.D. +0.1 -0.3
auxotrophy YNB Glucose N.D. -0.2 N.D. -0.2 +0.3
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 N.D. +0.0 -0.3
growth on oleic acid YNB Oleic Acid N.D. +0.2 N.D. -0.4 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.0 N.D. -0.1 +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.1 N.D. +0.0 -0.1
growth on ypd YPD N.D. -0.1 N.D. -0.3 +0.4
auxotrophy YNB Glucose 75mM Methionine N.D. -0.1 N.D. +0.0 +0.1
auxotrophy YNB Glucose 75mM Arginine N.D. -0.1 N.D. +0.0 +0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.1 N.D. -0.4 +0.6
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.1 N.D. -0.3 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.1 N.D. -0.5 +0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 N.D. +0.0 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.1 N.D. -0.2 +0.2
growth on ricinoleic acid YNB Glucose N.D. +0.1 N.D. -0.2 +0.4
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