Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11549 and 11550 are separated by 476 nucleotides11550 and 11551 are separated by 231 nucleotides11551 and 11552 are separated by 956 nucleotides11552 and 11553 are separated by 289 nucleotides mRNA_3181: 11549 - KOG4842 Protein involved in sister chromatid separation and/or segregation, at 30,988 to 32,695 11549 mRNA_3182: 11550 - KOG1575 Voltage-gated shaker-like K+ channel, subunit beta/KCNAB, at 33,172 to 34,723 11550 mRNA_3183: 11551 - HMMPfam-Cohesin loading factor-PF10345, at 34,955 to 37,755 11551 mRNA_3184: 11552 - K13220 WBP4, FBP21 WW domain-binding protein 4, at 38,712 to 39,951 11552 mRNA_3185: 11553 - KOG1577 Aldo/keto reductase family proteins, at 40,241 to 41,550 11553
Group Condition mRNA_3181 mRNA_3182 mRNA_3183 mRNA_3184 mRNA_3185
11549 11550 11551 11552 11553
growth on oleic acid YNB Oleic Acid -0.2 -0.1 N.D. N.D. -0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 -0.5 N.D. N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.1 -0.1 N.D. N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy +0.1 -0.1 N.D. N.D. -0.2
growth on oleic acid YNB Glucose +0.1 -0.1 N.D. N.D. -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.2 -0.2 N.D. N.D. -0.1
growth on ypd YPD -0.1 +0.1 N.D. N.D. +0.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 +0.2 N.D. N.D. -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 +0.1 N.D. N.D. -0.1
auxotrophy YNB Glucose 75mM Arginine +0.1 +0.0 N.D. N.D. +0.2
growth on ricinoleic acid YNB Glucose +0.2 +0.0 N.D. N.D. +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 +0.2 N.D. N.D. +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 +0.1 N.D. N.D. +0.1
auxotrophy YNB Glucose -0.0 +0.2 N.D. N.D. +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 +0.1 N.D. N.D. +0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.3 +0.1 N.D. N.D. +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 +0.0 N.D. N.D. +0.4
facs on lipid accumulation Sorted No BODIPY Gate +0.5 -0.0 N.D. N.D. +0.2
auxotrophy YNB Glucose 75mM Methionine +0.4 +0.1 N.D. N.D. +0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.4 +0.2 N.D. N.D. +0.2
auxotrophy Dropout Complete +0.5 +0.1 N.D. N.D. +0.2
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