Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11550 and 11551 are separated by 231 nucleotides11551 and 11552 are separated by 956 nucleotides11552 and 11553 are separated by 289 nucleotides11553 and 11554 are separated by 92 nucleotides mRNA_3182: 11550 - KOG1575 Voltage-gated shaker-like K+ channel, subunit beta/KCNAB, at 33,172 to 34,723 11550 mRNA_3183: 11551 - HMMPfam-Cohesin loading factor-PF10345, at 34,955 to 37,755 11551 mRNA_3184: 11552 - K13220 WBP4, FBP21 WW domain-binding protein 4, at 38,712 to 39,951 11552 mRNA_3185: 11553 - KOG1577 Aldo/keto reductase family proteins, at 40,241 to 41,550 11553 mRNA_3186: 11554 - KOG4444 Peroxisomal assembly protein PEX3, at 41,643 to 43,993 11554
Group Condition mRNA_3182 mRNA_3183 mRNA_3184 mRNA_3185 mRNA_3186
11550 11551 11552 11553 11554
ypd buoyancy 40 hrs No Buoyancy Separation -0.5 N.D. N.D. +0.1 N.D.
growth on oleic acid YNB Oleic Acid -0.1 N.D. N.D. -0.3 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.2 N.D. N.D. -0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.1 N.D. N.D. -0.2 N.D.
growth on oleic acid YNB Glucose -0.1 N.D. N.D. -0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. N.D. -0.1 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 N.D. N.D. -0.1 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 N.D. N.D. -0.1 N.D.
growth on ricinoleic acid YNB Glucose +0.0 N.D. N.D. +0.1 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.1 N.D. N.D. +0.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.0 N.D. N.D. +0.2 N.D.
growth on ypd YPD +0.1 N.D. N.D. +0.0 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 N.D. N.D. +0.1 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.0 N.D. N.D. +0.2 N.D.
auxotrophy Dropout Complete +0.1 N.D. N.D. +0.2 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 N.D. N.D. +0.1 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. N.D. +0.1 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 N.D. N.D. +0.1 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 N.D. N.D. +0.2 N.D.
auxotrophy YNB Glucose +0.2 N.D. N.D. +0.2 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 N.D. N.D. +0.4 N.D.
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