Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11552 and 11553 are separated by 289 nucleotides11553 and 11554 are separated by 92 nucleotides11554 and 11555 are separated by 444 nucleotides11555 and 11556 are separated by 271 nucleotides mRNA_3184: 11552 - K13220 WBP4, FBP21 WW domain-binding protein 4, at 38,712 to 39,951 11552 mRNA_3185: 11553 - KOG1577 Aldo/keto reductase family proteins, at 40,241 to 41,550 11553 mRNA_3186: 11554 - KOG4444 Peroxisomal assembly protein PEX3, at 41,643 to 43,993 11554 mRNA_3187: 11555 - BLAST Protein disulfide-isomerase erp38 [Hypsizygus marmoreus], at 44,438 to 45,168 11555 mRNA_3188: 11556 - Hypothetical Protein, at 45,440 to 45,769 11556
Group Condition mRNA_3184 mRNA_3185 mRNA_3186 mRNA_3187 mRNA_3188
11552 11553 11554 11555 11556
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.1 N.D. -0.7 -1.4
auxotrophy Dropout Complete N.D. +0.2 N.D. -0.6 -0.7
growth on oleic acid YNB Oleic Acid N.D. -0.3 N.D. -0.5 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.2 N.D. -1.0 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.1 N.D. -0.8 -0.1
growth on oleic acid YNB Glucose N.D. -0.1 N.D. -0.2 -0.5
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.1 N.D. +0.0 -0.5
auxotrophy YNB Glucose 75mM Arginine N.D. +0.2 N.D. -0.1 -0.5
auxotrophy YNB Glucose N.D. +0.2 N.D. -0.1 -0.5
growth on ricinoleic acid YNB Glucose N.D. +0.1 N.D. +0.1 -0.5
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 N.D. +0.6 -0.8
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.1 N.D. +0.2 -0.4
ypd buoyancy 40 hrs High Buoyancy N.D. -0.2 N.D. +0.5 -0.4
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 N.D. +0.4 -0.3
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.2 N.D. -0.1 -0.1
growth on ypd YPD N.D. +0.0 N.D. -0.6 +0.6
auxotrophy YNB Glucose 75mM Methionine N.D. +0.1 N.D. +0.2 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 N.D. +0.1 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.1 N.D. +0.6 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.4 N.D. -0.2 +0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.1 N.D. +0.4 +0.1
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