Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11566 and 11567 are separated by 256 nucleotides11567 and 11568 are separated by 637 nucleotides11568 and 11569 are separated by 199 nucleotides11569 and 11570 are separated by 476 nucleotides mRNA_3198: 11566 - K20176 SGSM3, MAP small G protein signaling modulator 3, at 66,778 to 71,121 11566 mRNA_3199: 11567 - K12881 THOC4, ALY THO complex subunit 4, at 71,378 to 73,355 11567 mRNA_3200: 11568 - KOG1515 Arylacetamide deacetylase, at 73,993 to 76,977 11568 mRNA_3201: 11569 - K12175 GPS1, COPS1, CSN1 COP9 signalosome complex subunit 1, at 77,177 to 79,098 11569 mRNA_3202: 11570 - KOG2533 Permease of the major facilitator superfamily, at 79,575 to 81,413 11570
Group Condition mRNA_3198 mRNA_3199 mRNA_3200 mRNA_3201 mRNA_3202
11566 11567 11568 11569 11570
facs on lipid accumulation Sorted High BODIPY Gate -0.4 N.D. -0.1 -1.4 +0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.4 N.D. +0.1 -1.3 -0.0
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 N.D. -0.3 -0.9 +0.3
growth on ypd YPD -0.2 N.D. -0.1 +0.1 -0.3
ypd buoyancy 40 hrs Low Buoyancy -0.4 N.D. -0.0 +0.0 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 N.D. -0.1 -0.4 -0.2
ypd buoyancy 40 hrs High Buoyancy +0.0 N.D. +0.0 -0.4 +0.2
growth on oleic acid YNB Glucose +0.0 N.D. -0.0 +0.1 -0.2
growth on oleic acid YNB Oleic Acid +0.2 N.D. +0.1 -0.1 -0.2
ypd buoyancy 40 hrs Median Buoyancy -0.0 N.D. -0.0 +0.2 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. -0.1 -0.3 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 N.D. -0.4 +0.9 -0.3
auxotrophy YNB Glucose 75mM Methionine -0.1 N.D. +0.2 -0.0 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 N.D. +0.1 +0.7 -0.2
growth on ricinoleic acid YNB Glucose +0.3 N.D. -0.0 +0.1 +0.1
auxotrophy YNB Glucose +0.0 N.D. +0.4 +0.1 -0.0
auxotrophy YNB Glucose 75mM Arginine -0.1 N.D. +0.2 +0.3 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 N.D. +0.1 -0.1 +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 N.D. +0.0 +0.6 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.4 N.D. +0.1 +0.1 +0.6
auxotrophy Dropout Complete +0.0 N.D. +0.2 +1.0 +0.2
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