Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11572 and 11573 are separated by 220 nucleotides11573 and 11574 are separated by 220 nucleotides11574 and 11575 are separated by 287 nucleotides11575 and 11576 are separated by 505 nucleotides mRNA_3204: 11572 - SUPERFAMILY--SSF69118, at 82,145 to 83,066 11572 mRNA_3205: 11573 - K13281 uvsE, UVE1 UV DNA damage endonuclease, at 83,287 to 85,713 11573 mRNA_3206: 11574 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 85,934 to 91,274 11574 mRNA_3207: 11575 - Hypothetical Protein, at 91,562 to 92,218 11575 mRNA_3208: 11576 - HMMPfam-Meiotically up-regulated gene 113-PF13455, at 92,724 to 94,778 11576
Group Condition mRNA_3204 mRNA_3205 mRNA_3206 mRNA_3207 mRNA_3208
11572 11573 11574 11575 11576
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 -0.5 -0.1 -0.1 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 +0.4 -0.4 +0.0 -0.2
growth on oleic acid YNB Oleic Acid -0.0 -0.3 -0.0 +0.1 -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.9 -0.2 +0.3 +0.8 -0.2
ypd buoyancy 40 hrs High Buoyancy -0.4 +0.2 -0.1 +0.3 -0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.2 +0.1 +0.3 +0.2 -0.4
growth on oleic acid YNB Glucose -0.1 +0.1 +0.1 -0.0 +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 -0.0 -0.2 -0.1 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 -0.1 -0.1 -0.2 +0.4
auxotrophy YNB Glucose 75mM Methionine +0.5 -0.1 -0.1 +0.1 +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 +0.1 -0.0 +0.3 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.7 -0.1 -0.2 +0.1 -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.3 +0.2 -0.2 +0.2 -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.3 +0.2 -0.1 +0.1 -0.0
auxotrophy YNB Glucose 75mM Arginine +0.5 +0.0 -0.1 +0.2 +0.1
auxotrophy YNB Glucose +0.7 -0.1 -0.1 +0.0 +0.0
growth on ricinoleic acid YNB Glucose +0.5 -0.1 +0.0 -0.1 +0.3
auxotrophy Dropout Complete +0.6 +0.1 -0.2 +0.3 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.7 +0.1 -0.1 +0.2 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.6 +0.1 +0.1 +0.3 +0.0
growth on ypd YPD +0.8 +0.1 +0.1 +0.1 +0.0
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