Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11587 and 11588 are separated by 192 nucleotides11588 and 11589 are separated by 1290 nucleotides11589 and 11590 are separated by 120 nucleotides11590 and 11591 are separated by 255 nucleotides mRNA_3219: 11587 - HMMPfam-RNase H-PF00075,ProSiteProfiles-RNase H domain profile.-PS50879,SUPERFAMILY--SSF53098, at 125,577 to 131,824 11587 mRNA_3220: 11588 - KOG0686 COP9 signalosome, subunit CSN1, at 132,017 to 133,130 11588 mRNA_3221: 11589 - KOG2533 Permease of the major facilitator superfamily, at 134,421 to 136,280 11589 mRNA_3222: 11590 - Hypothetical Protein, at 136,401 to 136,739 11590 mRNA_3223: 11591 - Hypothetical Protein, at 136,995 to 137,729 11591
Group Condition mRNA_3219 mRNA_3220 mRNA_3221 mRNA_3222 mRNA_3223
11587 11588 11589 11590 11591
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 -0.4 -0.5 N.D. -0.8
growth on ypd YPD +0.1 -0.3 -0.1 N.D. -0.9
facs on lipid accumulation Sorted No BODIPY Gate +0.0 -0.9 +0.2 N.D. -0.3
growth on ricinoleic acid YNB Glucose +0.1 -0.9 -0.1 N.D. -0.1
auxotrophy YNB Glucose +0.0 -0.7 -0.4 N.D. +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 -0.9 -0.2 N.D. +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 +0.1 -0.1 N.D. -0.5
auxotrophy Dropout Complete -0.0 -0.1 -0.5 N.D. +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 +0.6 -0.6 N.D. -0.4
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 -0.3 +0.1 N.D. -0.4
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.1 -0.3 N.D. +0.0
growth on oleic acid YNB Glucose +0.0 -0.2 -0.2 N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.2 -0.2 N.D. -0.2
auxotrophy YNB Glucose 75mM Arginine +0.1 -0.3 -0.5 N.D. +0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 +0.3 -0.4 N.D. -0.2
auxotrophy YNB Glucose 75mM Methionine +0.0 -0.2 -0.3 N.D. +0.4
growth on oleic acid YNB Oleic Acid +0.0 +0.4 -0.3 N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy -0.1 +0.4 -0.1 N.D. -0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 +0.3 +0.2 N.D. +0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.0 +0.2 +0.2 N.D. +0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 +0.2 +0.1 N.D. +0.3
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