Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11590 and 11591 are separated by 255 nucleotides11591 and 11592 are separated by 390 nucleotides11592 and 11593 are separated by 1002 nucleotides11593 and 11594 are separated by 232 nucleotides mRNA_3222: 11590 - Hypothetical Protein, at 136,401 to 136,739 11590 mRNA_3223: 11591 - Hypothetical Protein, at 136,995 to 137,729 11591 mRNA_3224: 11592 - BLAST UvdE-domain-containing protein [Gloeophyllum trab..., at 138,120 to 138,730 11592 mRNA_3225: 11593 - KOG0951 RNA helicase BRR2, DEAD-box superfamily, at 139,733 to 140,179 11593 mRNA_3226: 11594 - K05535 MNN2 alpha 1,2-mannosyltransferase, at 140,412 to 142,107 11594
Group Condition mRNA_3222 mRNA_3223 mRNA_3224 mRNA_3225 mRNA_3226
11590 11591 11592 11593 11594
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.8 N.D. -0.3 +0.0
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.3 N.D. -0.4 -0.7
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.5 N.D. +0.0 -0.0
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.4 N.D. -0.2 +0.1
growth on oleic acid YNB Oleic Acid N.D. -0.1 N.D. -0.0 -0.3
auxotrophy Dropout Complete N.D. +0.2 N.D. -0.6 -0.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.3 N.D. +0.0 -0.1
growth on ypd YPD N.D. -0.9 N.D. +0.6 +0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.2 N.D. -0.1 +0.2
growth on ricinoleic acid YNB Glucose N.D. -0.1 N.D. -0.5 +0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.4 N.D. +0.5 -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. +0.4 N.D. -0.1 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.3 N.D. -0.5 +0.2
auxotrophy YNB Glucose N.D. +0.3 N.D. -0.3 +0.0
ypd buoyancy 40 hrs High Buoyancy N.D. -0.2 N.D. +0.1 +0.2
growth on oleic acid YNB Glucose N.D. +0.1 N.D. +0.1 -0.2
auxotrophy YNB Glucose 75mM Arginine N.D. +0.5 N.D. -0.2 -0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.0 N.D. +0.3 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.2 N.D. +0.3 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.2 N.D. +0.0 +0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.2 N.D. +0.6 -0.2
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