Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11593 and 11594 are separated by 232 nucleotides11594 and 11595 are separated by 1035 nucleotides11595 and 11596 are separated by 421 nucleotides11596 and 11597 are separated by 229 nucleotides mRNA_3225: 11593 - KOG0951 RNA helicase BRR2, DEAD-box superfamily, at 139,733 to 140,179 11593 mRNA_3226: 11594 - K05535 MNN2 alpha 1,2-mannosyltransferase, at 140,412 to 142,107 11594 mRNA_3227: 11595 - K12854 SNRNP200, BRR2 pre-mRNA-splicing helicase BRR2, at 143,143 to 150,132 11595 mRNA_3228: 11596 - Hypothetical Protein, at 150,554 to 152,280 11596 mRNA_3229: 11597 - K01113 phoD alkaline phosphatase D, at 152,510 to 154,539 11597
Group Condition mRNA_3225 mRNA_3226 mRNA_3227 mRNA_3228 mRNA_3229
11593 11594 11595 11596 11597
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 -0.7 N.D. -0.0 +0.3
auxotrophy YNB Glucose 75mM Methionine -0.1 -0.3 N.D. -0.1 -0.2
auxotrophy Dropout Complete -0.6 -0.0 N.D. +0.1 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 +0.0 N.D. -0.1 -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 -0.0 N.D. -0.1 -0.2
growth on oleic acid YNB Oleic Acid -0.0 -0.3 N.D. -0.1 +0.1
growth on oleic acid YNB Glucose +0.1 -0.2 N.D. -0.0 -0.2
auxotrophy YNB Glucose -0.3 +0.0 N.D. +0.2 +0.0
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.2 N.D. -0.2 +0.1
auxotrophy YNB Glucose 75mM Arginine -0.2 -0.1 N.D. +0.2 +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.0 -0.1 N.D. +0.2 +0.0
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.2 N.D. -0.2 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.0 +0.2 N.D. +0.1 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.5 -0.1 N.D. +0.0 -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.3 -0.1 N.D. -0.1 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.5 +0.2 N.D. +0.2 +0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 +0.1 N.D. -0.0 -0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.6 -0.2 N.D. +0.3 -0.3
growth on ricinoleic acid YNB Glucose -0.5 +0.5 N.D. +0.1 +0.3
growth on ypd YPD +0.6 +0.0 N.D. +0.0 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 +0.1 N.D. +0.3 +0.4
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