Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11594 and 11595 are separated by 1035 nucleotides11595 and 11596 are separated by 421 nucleotides11596 and 11597 are separated by 229 nucleotides11597 and 11598 are separated by 940 nucleotides mRNA_3226: 11594 - K05535 MNN2 alpha 1,2-mannosyltransferase, at 140,412 to 142,107 11594 mRNA_3227: 11595 - K12854 SNRNP200, BRR2 pre-mRNA-splicing helicase BRR2, at 143,143 to 150,132 11595 mRNA_3228: 11596 - Hypothetical Protein, at 150,554 to 152,280 11596 mRNA_3229: 11597 - K01113 phoD alkaline phosphatase D, at 152,510 to 154,539 11597 mRNA_3230: 11598 - HMMPfam-Region of unknown function (DUF2417)-PF10329, at 155,480 to 157,759 11598
Group Condition mRNA_3226 mRNA_3227 mRNA_3228 mRNA_3229 mRNA_3230
11594 11595 11596 11597 11598
auxotrophy YNB Glucose 75mM Methionine -0.3 N.D. -0.1 -0.2 -0.2
auxotrophy Dropout Complete -0.0 N.D. +0.1 -0.1 -0.3
growth on oleic acid YNB Glucose -0.2 N.D. -0.0 -0.2 -0.0
facs on lipid accumulation Sorted Low BODIPY Gate -0.7 N.D. -0.0 +0.3 +0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.2 N.D. +0.3 -0.3 -0.1
growth on oleic acid YNB Oleic Acid -0.3 N.D. -0.1 +0.1 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 N.D. +0.0 -0.1 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 N.D. -0.1 -0.2 +0.2
auxotrophy YNB Glucose 75mM Arginine -0.1 N.D. +0.2 +0.1 -0.2
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. -0.1 +0.1 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. -0.0 -0.1 +0.0
auxotrophy YNB Glucose +0.0 N.D. +0.2 +0.0 -0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.1 N.D. +0.2 +0.0 +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 N.D. -0.1 -0.0 +0.2
growth on ypd YPD +0.0 N.D. +0.0 -0.2 +0.3
ypd buoyancy 40 hrs High Buoyancy +0.2 N.D. -0.2 +0.1 +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.2 N.D. -0.2 +0.1 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 N.D. +0.1 -0.0 +0.2
growth on ricinoleic acid YNB Glucose +0.5 N.D. +0.1 +0.3 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 N.D. +0.2 +0.5 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 N.D. +0.3 +0.4 -0.0
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