Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11595 and 11596 are separated by 421 nucleotides11596 and 11597 are separated by 229 nucleotides11597 and 11598 are separated by 940 nucleotides11598 and 11599 are separated by 42 nucleotides mRNA_3227: 11595 - K12854 SNRNP200, BRR2 pre-mRNA-splicing helicase BRR2, at 143,143 to 150,132 11595 mRNA_3228: 11596 - Hypothetical Protein, at 150,554 to 152,280 11596 mRNA_3229: 11597 - K01113 phoD alkaline phosphatase D, at 152,510 to 154,539 11597 mRNA_3230: 11598 - HMMPfam-Region of unknown function (DUF2417)-PF10329, at 155,480 to 157,759 11598 mRNA_3231: 11599 - KOG1430 C-3 sterol dehydrogenase/3-beta-hydroxysteroid dehydrogenase and related dehydrogenases, at 157,802 to 159,637 11599
Group Condition mRNA_3227 mRNA_3228 mRNA_3229 mRNA_3230 mRNA_3231
11595 11596 11597 11598 11599
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.3 -0.3 -0.1 -0.4
auxotrophy YNB Glucose 75mM Methionine N.D. -0.1 -0.2 -0.2 -0.1
auxotrophy Dropout Complete N.D. +0.1 -0.1 -0.3 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.0 -0.1 +0.0 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.0 -0.1 +0.0 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.1 -0.2 +0.2 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.1 -0.0 +0.2 -0.3
growth on ypd YPD N.D. +0.0 -0.2 +0.3 -0.3
growth on oleic acid YNB Glucose N.D. -0.0 -0.2 -0.0 +0.1
growth on ricinoleic acid YNB Glucose N.D. +0.1 +0.3 -0.2 -0.2
auxotrophy YNB Glucose N.D. +0.2 +0.0 -0.2 -0.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.2 +0.0 +0.0 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.2 +0.5 -0.1 -0.5
growth on oleic acid YNB Oleic Acid N.D. -0.1 +0.1 +0.1 +0.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.1 -0.0 +0.2 -0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 +0.1 +0.1 +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. -0.2 +0.1 +0.1 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.3 +0.4 -0.0 -0.3
auxotrophy YNB Glucose 75mM Arginine N.D. +0.2 +0.1 -0.2 +0.3
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.2 +0.1 +0.2 +0.3
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.0 +0.3 +0.1 +0.4
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