Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11600 and 11601 are separated by 423 nucleotides11601 and 11602 are separated by 181 nucleotides11602 and 11603 are separated by 4037 nucleotides11603 and 11604 are separated by 240 nucleotides mRNA_3232: 11600 - ProSiteProfiles-BRCT domain profile.-PS50172, at 159,953 to 161,045 11600 mRNA_3233: 11601 - KOG1339 Aspartyl protease, at 161,469 to 163,797 11601 mRNA_3234: 11602 - Hypothetical Protein, at 163,979 to 166,240 11602 mRNA_3235: 11603 - Hypothetical Protein, at 170,278 to 173,457 11603 mRNA_3236: 11604 - K01755 argH, ASL argininosuccinate lyase, at 173,698 to 175,795 11604
Group Condition mRNA_3232 mRNA_3233 mRNA_3234 mRNA_3235 mRNA_3236
11600 11601 11602 11603 11604
growth on oleic acid YNB Glucose -1.3 +0.0 -0.4 +0.2 -5.9
facs on lipid accumulation Sorted No BODIPY Gate +0.6 -0.1 -0.5 -0.3 -6.3
auxotrophy YNB Glucose -0.3 +0.1 -0.0 -0.0 -5.9
auxotrophy YNB Glucose 75mM Methionine -0.5 -0.0 -0.3 -0.1 -4.9
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.5 -0.1 -0.1 -0.1 -5.9
growth on ricinoleic acid YNB Ricinoleic Acid +1.4 +0.1 -0.7 -0.2 -5.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy +1.2 +0.1 +0.1 -0.2 -6.0
growth on oleic acid YNB Oleic Acid -0.4 +0.1 -0.8 -0.2 -3.3
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 -0.2 -0.9 +0.1 -3.5
growth on ricinoleic acid YNB Methylricinoleic Acid +1.5 +0.3 -0.3 -0.2 -5.7
buoyancy on lipid accumulation 40 hrs High Buoyancy +1.6 -0.4 -0.2 -0.1 -3.6
growth on ricinoleic acid YNB Glucose +1.8 +0.1 +0.1 -0.1 -4.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +1.8 -0.1 -0.1 -0.3 -3.4
facs on lipid accumulation Sorted High BODIPY Gate +0.6 -0.6 -0.4 +0.2 -1.9
auxotrophy Dropout Complete -0.8 -0.0 -0.5 +0.0 -0.4
ypd buoyancy 40 hrs No Buoyancy Separation -1.0 -0.9 -0.0 +0.2 +0.0
auxotrophy YNB Glucose 75mM Arginine -0.9 -0.1 -0.1 -0.1 -0.4
ypd buoyancy 40 hrs High Buoyancy +0.7 -0.1 +0.2 +0.1 +0.3
growth on ypd YPD +1.8 -0.1 +0.1 -0.3 -0.2
ypd buoyancy 40 hrs Median Buoyancy +1.4 -0.2 +0.1 +0.0 +0.1
ypd buoyancy 40 hrs Low Buoyancy +1.5 -0.1 +0.0 +0.1 +0.5
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