Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11609 and 11610 are separated by 114 nucleotides11610 and 11611 are separated by 385 nucleotides11611 and 11612 are separated by 849 nucleotides11612 and 11613 are separated by 459 nucleotides mRNA_3241: 11609 - SUPERFAMILY--SSF52047, at 192,497 to 194,768 11609 mRNA_3242: 11610 - K01814 hisA phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase, at 194,883 to 196,226 11610 mRNA_3243: 11611 - K10752 RBBP4, HAT2, CAF1, MIS16 histone-binding protein RBBP4, at 196,612 to 198,456 11611 mRNA_3244: 11612 - Hypothetical Protein, at 199,306 to 200,606 11612 mRNA_3245: 11613 - HMMPfam-Fungal protein of unknown function (DUF1752)-PF08550,HMMPfam-Protein of unknown function (DUF3295)-PF11702, at 201,066 to 204,037 11613
Group Condition mRNA_3241 mRNA_3242 mRNA_3243 mRNA_3244 mRNA_3245
11609 11610 11611 11612 11613
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 N.D. N.D. -1.9 -0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. N.D. -0.5 -1.7
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 N.D. N.D. -0.2 -1.6
growth on oleic acid YNB Glucose +0.2 N.D. N.D. -0.0 -0.7
growth on ricinoleic acid YNB Glucose +0.2 N.D. N.D. +0.0 -0.8
auxotrophy Dropout Complete -0.2 N.D. N.D. -0.3 +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.2 N.D. N.D. +0.2 -0.4
auxotrophy YNB Glucose -0.2 N.D. N.D. +0.2 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 N.D. N.D. -0.5 +0.0
auxotrophy YNB Glucose 75mM Methionine -0.1 N.D. N.D. -0.3 +0.5
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 N.D. N.D. +2.0 -1.8
growth on ypd YPD -0.1 N.D. N.D. +0.1 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 N.D. N.D. -0.6 +0.9
auxotrophy YNB Glucose 75mM Arginine +0.2 N.D. N.D. +0.3 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 N.D. N.D. +0.6 -0.0
ypd buoyancy 40 hrs High Buoyancy -0.1 N.D. N.D. +0.4 +0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.2 N.D. N.D. +1.9 -1.4
facs on lipid accumulation Sorted High BODIPY Gate -0.1 N.D. N.D. +1.3 -0.2
ypd buoyancy 40 hrs Low Buoyancy -0.2 N.D. N.D. +0.8 +0.7
growth on oleic acid YNB Oleic Acid -0.1 N.D. N.D. +0.7 +1.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 N.D. N.D. +0.2 +1.4
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