Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11617 and 11618 are separated by 155 nucleotides11618 and 11619 are separated by 246 nucleotides11619 and 11620 are separated by 137 nucleotides11620 and 11621 are separated by 408 nucleotides mRNA_3249: 11617 - Hypothetical Protein, at 211,752 to 212,300 11617 mRNA_3250: 11618 - K00415 QCR2, UQCRC2 ubiquinol-cytochrome c reductase core subunit 2, at 212,456 to 214,047 11618 mRNA_3251: 11619 - K19986 EXOC8, SEC84 exocyst complex component 8, at 214,294 to 217,210 11619 mRNA_3252: 11620 - KOG3348 BolA (bacterial stress-induced morphogen)-related protein, at 217,348 to 218,013 11620 mRNA_3253: 11621 - KOG1201 Hydroxysteroid 17-beta dehydrogenase 11, at 218,422 to 220,105 11621
Group Condition mRNA_3249 mRNA_3250 mRNA_3251 mRNA_3252 mRNA_3253
11617 11618 11619 11620 11621
ypd buoyancy 40 hrs No Buoyancy Separation -1.1 N.D. -2.2 -0.6 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 N.D. -1.7 -0.3 -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.2 N.D. -1.4 -0.1 -0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.0 N.D. -1.3 +0.5 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 N.D. +0.5 -0.5 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 N.D. -0.2 -0.2 +0.2
auxotrophy YNB Glucose +0.0 N.D. -0.6 +0.1 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.3 N.D. +0.8 -0.9 +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.2 N.D. +0.0 -0.2 +0.1
growth on oleic acid YNB Oleic Acid +0.2 N.D. +0.2 -0.4 +0.0
growth on ricinoleic acid YNB Glucose +0.2 N.D. +0.2 -0.7 +0.4
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 N.D. +0.2 -0.3 +0.4
auxotrophy YNB Glucose 75mM Arginine -0.1 N.D. -0.1 +0.3 +0.2
auxotrophy YNB Glucose 75mM Methionine -0.1 N.D. +0.0 +0.4 +0.1
ypd buoyancy 40 hrs Low Buoyancy -0.0 N.D. +0.2 +0.2 +0.1
ypd buoyancy 40 hrs High Buoyancy -0.1 N.D. +0.1 +0.3 +0.2
growth on ypd YPD +0.0 N.D. +0.6 +0.1 -0.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 N.D. +0.7 -0.6 +0.5
auxotrophy Dropout Complete +0.5 N.D. -0.4 +0.7 +0.2
growth on oleic acid YNB Glucose +0.2 N.D. +0.9 -0.4 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.5 N.D. +1.8 -0.3 +0.0
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