Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11624 and 11625 are separated by 356 nucleotides11625 and 11626 are separated by 44 nucleotides11626 and 11627 are separated by 143 nucleotides11627 and 11628 are separated by 195 nucleotides mRNA_3256: 11624 - K01681 ACO, acnA aconitate hydratase, at 224,964 to 230,364 11624 mRNA_3257: 11625 - K13869 SLC7A11 solute carrier family 7 (L-type amino acid transporter), member 11, at 230,721 to 232,745 11625 mRNA_3258: 11626 - K00791 miaA, TRIT1 tRNA dimethylallyltransferase, at 232,790 to 234,851 11626 mRNA_3259: 11627 - Hypothetical Protein, at 234,995 to 236,136 11627 mRNA_3260: 11628 - HMMPfam-Eukaryotic mitochondrial regulator protein-PF12298, at 236,332 to 237,668 11628
Group Condition mRNA_3256 mRNA_3257 mRNA_3258 mRNA_3259 mRNA_3260
11624 11625 11626 11627 11628
ypd buoyancy 40 hrs Median Buoyancy -0.2 -0.1 N.D. -0.2 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.0 -0.0 N.D. -0.4 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 -0.1 N.D. -0.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 -0.2 N.D. -0.1 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.0 +0.0 N.D. -0.3 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.3 -0.0 N.D. +0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.2 -0.1 N.D. +0.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.1 +0.0 N.D. -0.2 N.D.
growth on ypd YPD +0.0 +0.1 N.D. -0.2 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.0 -0.0 N.D. -0.0 N.D.
auxotrophy Dropout Complete -0.0 +0.2 N.D. +0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 +0.0 N.D. -0.0 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 +0.0 N.D. +0.1 N.D.
auxotrophy YNB Glucose +0.1 +0.0 N.D. +0.2 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.1 +0.3 N.D. -0.1 N.D.
growth on oleic acid YNB Glucose -0.1 +0.1 N.D. +0.3 N.D.
growth on ricinoleic acid YNB Glucose +0.2 +0.1 N.D. +0.4 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 +0.2 N.D. +0.4 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.5 -0.2 N.D. +0.5 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 +0.0 N.D. +0.6 N.D.
growth on oleic acid YNB Oleic Acid +0.2 +0.1 N.D. +0.7 N.D.
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