Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11642 and 11643 are separated by 57 nucleotides11643 and 11644 are separated by 188 nucleotides11644 and 11645 are separated by 724 nucleotides11645 and 11646 are separated by 244 nucleotides mRNA_3274: 11642 - K00108 betA, CHDH choline dehydrogenase, at 263,216 to 265,708 11642 mRNA_3275: 11643 - BLAST methyltransferase [Obba rivulosa], at 265,766 to 267,917 11643 mRNA_3276: 11644 - BLAST Nuclease PA3 [Grifola frondosa], at 268,106 to 268,881 11644 mRNA_3277: 11645 - K14835 NOP2 ribosomal RNA methyltransferase Nop2, at 269,606 to 272,171 11645 mRNA_3278: 11646 - K14152 HIS4 phosphoribosyl-ATP pyrophosphohydrolase / phosphoribosyl-AMP cyclohydrolase / histidinol dehydrogenase, at 272,416 to 277,579 11646
Group Condition mRNA_3274 mRNA_3275 mRNA_3276 mRNA_3277 mRNA_3278
11642 11643 11644 11645 11646
ypd buoyancy 40 hrs No Buoyancy Separation -0.6 -0.0 -0.5 N.D. -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 -0.3 +0.1 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 -0.1 +0.0 N.D. +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 -0.2 -0.0 N.D. +0.1
growth on oleic acid YNB Oleic Acid -0.1 -0.1 -0.1 N.D. +0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.4 -0.3 +0.2 N.D. +0.5
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 -0.1 +0.2 N.D. -0.0
growth on oleic acid YNB Glucose +0.0 -0.0 +0.0 N.D. -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.0 -0.1 -0.1 N.D. +0.2
growth on ricinoleic acid YNB Glucose +0.1 +0.0 -0.2 N.D. +0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 -0.1 +0.0 N.D. +0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.0 -0.2 +0.4 N.D. +0.0
ypd buoyancy 40 hrs High Buoyancy +0.4 +0.2 -0.0 N.D. -0.3
ypd buoyancy 40 hrs Median Buoyancy +0.4 +0.1 +0.0 N.D. -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 +0.1 -0.2 N.D. +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.3 +0.1 +0.1 N.D. -0.1
auxotrophy Dropout Complete +0.1 +0.1 +0.1 N.D. +0.3
auxotrophy YNB Glucose 75mM Arginine +0.0 +0.2 +0.1 N.D. +0.3
growth on ypd YPD +0.5 +0.1 +0.1 N.D. -0.0
auxotrophy YNB Glucose +0.1 +0.2 +0.0 N.D. +0.4
auxotrophy YNB Glucose 75mM Methionine +0.0 +0.2 +0.3 N.D. +0.4
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