Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11645 and 11646 are separated by 244 nucleotides11646 and 11647 are separated by 733 nucleotides11647 and 11648 are separated by 1011 nucleotides11648 and 11649 are separated by 215 nucleotides mRNA_3277: 11645 - K14835 NOP2 ribosomal RNA methyltransferase Nop2, at 269,606 to 272,171 11645 mRNA_3278: 11646 - K14152 HIS4 phosphoribosyl-ATP pyrophosphohydrolase / phosphoribosyl-AMP cyclohydrolase / histidinol dehydrogenase, at 272,416 to 277,579 11646 mRNA_3279: 11647 - HMMPfam-Beta-glucan synthesis-associated protein (SKN1)-PF03935,SUPERFAMILY--SSF49899, at 278,313 to 280,858 11647 mRNA_3280: 11648 - Hypothetical Protein, at 281,870 to 282,355 11648 mRNA_3281: 11649 - KOG0255 Synaptic vesicle transporter SVOP and related transporters (major facilitator superfamily), at 282,571 to 284,691 11649
Group Condition mRNA_3277 mRNA_3278 mRNA_3279 mRNA_3280 mRNA_3281
11645 11646 11647 11648 11649
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.2 N.D. -0.2 -0.1
growth on ypd YPD N.D. -0.0 N.D. +0.1 -0.4
ypd buoyancy 40 hrs High Buoyancy N.D. -0.3 N.D. -0.1 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.1 N.D. -0.0 -0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 N.D. -0.2 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.1 N.D. +0.3 -0.1
auxotrophy Dropout Complete N.D. +0.3 N.D. -0.2 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.0 N.D. +0.5 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.2 N.D. +0.1 +0.0
auxotrophy YNB Glucose 75mM Methionine N.D. +0.4 N.D. -0.1 +0.1
growth on oleic acid YNB Oleic Acid N.D. +0.2 N.D. -0.0 +0.2
auxotrophy YNB Glucose N.D. +0.4 N.D. -0.2 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.1 N.D. +0.1 +0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.1 N.D. +0.2 +0.2
growth on ricinoleic acid YNB Glucose N.D. +0.1 N.D. +0.2 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.3 N.D. +0.6 -0.3
auxotrophy YNB Glucose 75mM Arginine N.D. +0.3 N.D. -0.2 +0.3
growth on oleic acid YNB Glucose N.D. -0.1 N.D. +0.1 +0.6
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.0 N.D. +0.5 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 N.D. +0.2 +0.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.5 N.D. +0.3 +0.2
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