Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11646 and 11647 are separated by 733 nucleotides11647 and 11648 are separated by 1011 nucleotides11648 and 11649 are separated by 215 nucleotides11649 and 11650 are separated by 475 nucleotides mRNA_3278: 11646 - K14152 HIS4 phosphoribosyl-ATP pyrophosphohydrolase / phosphoribosyl-AMP cyclohydrolase / histidinol dehydrogenase, at 272,416 to 277,579 11646 mRNA_3279: 11647 - HMMPfam-Beta-glucan synthesis-associated protein (SKN1)-PF03935,SUPERFAMILY--SSF49899, at 278,313 to 280,858 11647 mRNA_3280: 11648 - Hypothetical Protein, at 281,870 to 282,355 11648 mRNA_3281: 11649 - KOG0255 Synaptic vesicle transporter SVOP and related transporters (major facilitator superfamily), at 282,571 to 284,691 11649 mRNA_3282: 11650 - KOG0023 Alcohol dehydrogenase, class V, at 285,167 to 286,436 11650
Group Condition mRNA_3278 mRNA_3279 mRNA_3280 mRNA_3281 mRNA_3282
11646 11647 11648 11649 11650
ypd buoyancy 40 hrs Median Buoyancy -0.2 N.D. -0.2 -0.1 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 N.D. -0.0 -0.2 -0.6
growth on ypd YPD -0.0 N.D. +0.1 -0.4 +0.1
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. -0.2 +0.3 -0.1
ypd buoyancy 40 hrs High Buoyancy -0.3 N.D. -0.1 +0.1 +0.3
auxotrophy Dropout Complete +0.3 N.D. -0.2 +0.1 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 N.D. +0.3 -0.1 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 N.D. +0.2 +0.2 -0.4
auxotrophy YNB Glucose 75mM Methionine +0.4 N.D. -0.1 +0.1 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 N.D. +0.1 +0.5 -0.3
auxotrophy YNB Glucose 75mM Arginine +0.3 N.D. -0.2 +0.3 -0.2
auxotrophy YNB Glucose +0.4 N.D. -0.2 +0.2 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 N.D. +0.1 +0.0 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. +0.2 +0.3 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 N.D. +0.6 -0.3 -0.0
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 N.D. +0.5 -0.3 +0.3
growth on oleic acid YNB Oleic Acid +0.2 N.D. -0.0 +0.2 +0.2
growth on ricinoleic acid YNB Glucose +0.1 N.D. +0.2 +0.2 +0.2
growth on oleic acid YNB Glucose -0.1 N.D. +0.1 +0.6 +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.0 N.D. +0.5 +0.1 +0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.5 N.D. +0.3 +0.2 +0.4
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