Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11654 and 11655 are separated by 269 nucleotides11655 and 11656 are separated by 189 nucleotides11656 and 11657 are separated by 296 nucleotides11657 and 11658 are separated by 2423 nucleotides mRNA_3286: 11654 - Hypothetical Protein, at 297,200 to 297,720 11654 mRNA_3287: 11655 - Hypothetical Protein, at 297,990 to 298,361 11655 mRNA_3288: 11656 - K03240 EIF2B5 translation initiation factor eIF-2B subunit epsilon, at 298,551 to 301,179 11656 mRNA_3289: 11657 - ProSiteProfiles-Prokaryotic membrane lipoprotein lipid attachment site profile.-PS51257, at 301,476 to 302,545 11657 mRNA_3290: 11658 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 304,969 to 308,540 11658
Group Condition mRNA_3286 mRNA_3287 mRNA_3288 mRNA_3289 mRNA_3290
11654 11655 11656 11657 11658
facs on lipid accumulation Sorted No BODIPY Gate -0.2 -2.2 N.D. N.D. -0.4
growth on ricinoleic acid YNB Glucose -1.4 -0.7 N.D. N.D. -0.5
growth on oleic acid YNB Oleic Acid -1.0 -1.3 N.D. N.D. -0.3
ypd buoyancy 40 hrs No Buoyancy Separation -1.5 -1.1 N.D. N.D. +0.2
growth on ricinoleic acid YNB Ricinoleic Acid -1.0 -1.0 N.D. N.D. -0.4
growth on oleic acid YNB Glucose -0.5 -0.9 N.D. N.D. -0.7
auxotrophy YNB Glucose 75mM Arginine -0.3 -0.7 N.D. N.D. -0.6
auxotrophy YNB Glucose -0.6 -0.4 N.D. N.D. -0.3
ypd buoyancy 40 hrs Low Buoyancy -0.6 -0.7 N.D. N.D. +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 -0.6 N.D. N.D. -0.3
auxotrophy Dropout Complete +0.0 -1.0 N.D. N.D. -0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.0 -0.9 N.D. N.D. -0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 -0.1 N.D. N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy -0.5 -0.0 N.D. N.D. +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.0 -0.5 N.D. N.D. +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +1.9 -0.6 N.D. N.D. -1.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +1.9 -1.4 N.D. N.D. -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +1.7 -0.7 N.D. N.D. -0.5
growth on ypd YPD +0.2 +0.6 N.D. N.D. +0.1
auxotrophy YNB Glucose 75mM Methionine +1.1 +0.5 N.D. N.D. -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +1.9 +0.1 N.D. N.D. -0.7
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