Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11656 and 11657 are separated by 296 nucleotides11657 and 11658 are separated by 2423 nucleotides11658 and 11659 are separated by 364 nucleotides11659 and 11660 are separated by 584 nucleotides mRNA_3288: 11656 - K03240 EIF2B5 translation initiation factor eIF-2B subunit epsilon, at 298,551 to 301,179 11656 mRNA_3289: 11657 - ProSiteProfiles-Prokaryotic membrane lipoprotein lipid attachment site profile.-PS51257, at 301,476 to 302,545 11657 mRNA_3290: 11658 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 304,969 to 308,540 11658 mRNA_3291: 11659 - BLAST expressed protein [Schizophyllum commune H4-8], at 308,905 to 310,428 11659 mRNA_3292: 11660 - KOG1339 Aspartyl protease, at 311,013 to 311,790 11660
Group Condition mRNA_3288 mRNA_3289 mRNA_3290 mRNA_3291 mRNA_3292
11656 11657 11658 11659 11660
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.7 +0.0 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -1.0 +0.2 -0.1
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.6 -0.2 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.5 +0.1 -0.2
growth on oleic acid YNB Glucose N.D. N.D. -0.7 -0.0 +0.2
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.4 -0.0 +0.1
auxotrophy YNB Glucose N.D. N.D. -0.3 +0.2 -0.0
auxotrophy Dropout Complete N.D. N.D. -0.1 +0.0 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -0.1 -0.1 +0.3
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.5 +0.3 +0.3
growth on ypd YPD N.D. N.D. +0.1 -0.0 +0.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.4 +0.2 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.3 +0.2 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.2 +0.3 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.4 +0.3 +0.3
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.3 +0.4 +0.1
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.2 +0.1 -0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.2 +0.2 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. +0.2 +0.0 +0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.2 +0.3 +0.3
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. +0.1 +0.2 +0.2
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