Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11657 and 11658 are separated by 2423 nucleotides11658 and 11659 are separated by 364 nucleotides11659 and 11660 are separated by 584 nucleotides11660 and 11661 are separated by 6412 nucleotides mRNA_3289: 11657 - ProSiteProfiles-Prokaryotic membrane lipoprotein lipid attachment site profile.-PS51257, at 301,476 to 302,545 11657 mRNA_3290: 11658 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 304,969 to 308,540 11658 mRNA_3291: 11659 - BLAST expressed protein [Schizophyllum commune H4-8], at 308,905 to 310,428 11659 mRNA_3292: 11660 - KOG1339 Aspartyl protease, at 311,013 to 311,790 11660 mRNA_3293: 11661 - K01440 PNC1 nicotinamidase, at 318,203 to 319,692 11661
Group Condition mRNA_3289 mRNA_3290 mRNA_3291 mRNA_3292 mRNA_3293
11657 11658 11659 11660 11661
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -1.0 +0.2 -0.1 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.7 +0.0 -0.3 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.5 +0.1 -0.2 -0.2
auxotrophy YNB Glucose 75mM Arginine N.D. -0.6 -0.2 -0.1 +0.3
growth on oleic acid YNB Glucose N.D. -0.7 -0.0 +0.2 +0.2
growth on ricinoleic acid YNB Glucose N.D. -0.5 +0.3 +0.3 -0.4
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.4 +0.3 +0.3 -0.3
growth on oleic acid YNB Oleic Acid N.D. -0.3 +0.4 +0.1 -0.3
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.1 -0.1 +0.3 -0.1
growth on ypd YPD N.D. +0.1 -0.0 +0.0 -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. -0.4 -0.0 +0.1 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.3 +0.2 +0.3 -0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.2 +0.3 +0.3 -0.4
auxotrophy Dropout Complete N.D. -0.1 +0.0 -0.1 +0.2
auxotrophy YNB Glucose N.D. -0.3 +0.2 -0.0 +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. +0.2 +0.1 -0.0 -0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.4 +0.2 +0.3 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.2 +0.3 +0.1 +0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.2 +0.2 -0.0 -0.0
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.1 +0.2 +0.2 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.2 +0.0 +0.2 +0.2
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