Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11669 and 11670 are separated by 1362 nucleotides11670 and 11671 are separated by 4994 nucleotides11671 and 11672 are separated by 201 nucleotides11672 and 11673 are separated by 807 nucleotides mRNA_3301: 11669 - K08197 ARN MFS transporter, SIT family, siderophore-iron-H+ symporter, at 339,708 to 341,858 11669 mRNA_3302: 11670 - K07232 chaC cation transport protein ChaC, at 343,221 to 344,290 11670 mRNA_3303: 11671 - K03249 EIF3F translation initiation factor 3 subunit F, at 349,285 to 350,385 11671 mRNA_3304: 11672 - KOG2753 Uncharacterized conserved protein, contains PCI domain, at 350,587 to 352,119 11672 mRNA_3305: 11673 - KOG3299 Uncharacterized conserved protein, at 352,927 to 353,808 11673
Group Condition mRNA_3301 mRNA_3302 mRNA_3303 mRNA_3304 mRNA_3305
11669 11670 11671 11672 11673
facs on lipid accumulation Sorted Low BODIPY Gate -0.5 +0.4 N.D. N.D. -2.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.0 N.D. N.D. -0.8
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 -0.2 N.D. N.D. -0.9
growth on oleic acid YNB Glucose -0.0 +0.8 N.D. N.D. -0.8
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 -0.1 N.D. N.D. -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 -0.1 N.D. N.D. +0.0
facs on lipid accumulation Sorted High BODIPY Gate +0.3 +1.3 N.D. N.D. -1.6
ypd buoyancy 40 hrs Median Buoyancy +0.0 -0.0 N.D. N.D. +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 +0.4 N.D. N.D. -0.1
growth on ypd YPD -0.4 +0.5 N.D. N.D. +0.1
growth on oleic acid YNB Oleic Acid -0.1 +1.6 N.D. N.D. -0.8
facs on lipid accumulation Sorted No BODIPY Gate -0.0 +0.3 N.D. N.D. +0.5
ypd buoyancy 40 hrs High Buoyancy +0.0 +0.4 N.D. N.D. +0.5
ypd buoyancy 40 hrs Low Buoyancy +0.3 +0.6 N.D. N.D. -0.0
auxotrophy YNB Glucose -0.0 +0.9 N.D. N.D. +0.7
growth on ricinoleic acid YNB Glucose -0.1 +1.1 N.D. N.D. +0.6
auxotrophy Dropout Complete -0.1 +1.1 N.D. N.D. +1.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 +2.5 N.D. N.D. +0.2
auxotrophy YNB Glucose 75mM Methionine +0.0 +1.0 N.D. N.D. +1.7
auxotrophy YNB Glucose 75mM Arginine +0.1 +1.1 N.D. N.D. +2.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +2.1 N.D. N.D. +1.2
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