Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11671 and 11672 are separated by 201 nucleotides11672 and 11673 are separated by 807 nucleotides11673 and 11674 are separated by 13 nucleotides11674 and 11675 are separated by 249 nucleotides mRNA_3303: 11671 - K03249 EIF3F translation initiation factor 3 subunit F, at 349,285 to 350,385 11671 mRNA_3304: 11672 - KOG2753 Uncharacterized conserved protein, contains PCI domain, at 350,587 to 352,119 11672 mRNA_3305: 11673 - KOG3299 Uncharacterized conserved protein, at 352,927 to 353,808 11673 mRNA_3306: 11674 - KOG0845 Nuclear pore complex, Nup98 component (sc Nup145/Nup100/Nup116), at 353,822 to 355,437 11674 mRNA_3307: 11675 - K06268 PPP3R, CNB serine/threonine-protein phosphatase 2B regulatory subunit, at 355,687 to 356,880 11675
Group Condition mRNA_3303 mRNA_3304 mRNA_3305 mRNA_3306 mRNA_3307
11671 11672 11673 11674 11675
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -2.5 N.D. -1.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.9 N.D. -1.4
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -1.6 N.D. +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.4 N.D. -0.5
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.2 N.D. -0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -0.8 N.D. +0.8
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.5 N.D. -0.6
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.5 N.D. -0.5
growth on ypd YPD N.D. N.D. +0.1 N.D. +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.1 N.D. +0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.0 N.D. +0.5
growth on oleic acid YNB Glucose N.D. N.D. -0.8 N.D. +1.6
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.0 N.D. +0.9
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.8 N.D. +2.4
auxotrophy YNB Glucose N.D. N.D. +0.7 N.D. +1.9
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.6 N.D. +2.3
auxotrophy Dropout Complete N.D. N.D. +1.2 N.D. +1.9
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +1.7 N.D. +1.6
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +0.2 N.D. +3.4
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. +2.2 N.D. +1.8
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +1.2 N.D. +3.6
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