Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11700 and 11701 are separated by 152 nucleotides11701 and 11702 are separated by 101 nucleotides11702 and 11703 are separated by 114 nucleotides11703 and 11704 are separated by 139 nucleotides mRNA_3332: 11700 - K17424 MRPL43 large subunit ribosomal protein L43, at 411,009 to 411,517 11700 mRNA_3333: 11701 - K05283 PIGW phosphatidylinositol glycan, class W, at 411,670 to 413,695 11701 mRNA_3334: 11702 - K03936 NDUFS3 NADH dehydrogenase (ubiquinone) Fe-S protein 3, at 413,797 to 414,963 11702 mRNA_3335: 11703 - Hypothetical Protein, at 415,078 to 415,551 11703 mRNA_3336: 11704 - K07827 KRAS, KRAS2 GTPase KRas, at 415,691 to 416,703 11704
Group Condition mRNA_3332 mRNA_3333 mRNA_3334 mRNA_3335 mRNA_3336
11700 11701 11702 11703 11704
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. N.D. -0.3 -0.3
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. N.D. -0.6 +0.0
growth on oleic acid YNB Oleic Acid N.D. N.D. N.D. -0.1 -0.3
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. N.D. -0.1 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. N.D. -0.1 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. N.D. -0.3 +0.1
growth on oleic acid YNB Glucose N.D. N.D. N.D. +0.0 -0.2
growth on ypd YPD N.D. N.D. N.D. +0.1 -0.3
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. N.D. -0.1 -0.1
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. N.D. -0.1 +0.0
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. N.D. -0.2 +0.2
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. N.D. +0.4 -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. N.D. +0.1 -0.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. N.D. +0.5 -0.3
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. N.D. +0.3 -0.1
auxotrophy YNB Glucose N.D. N.D. N.D. +0.6 -0.2
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. N.D. +0.4 -0.0
auxotrophy Dropout Complete N.D. N.D. N.D. +0.6 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. N.D. +0.3 +0.0
growth on ricinoleic acid YNB Glucose N.D. N.D. N.D. +0.3 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. N.D. +0.4 +0.1
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