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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
11700 and 11701 are separated by 152 nucleotides
11701 and 11702 are separated by 101 nucleotides
11702 and 11703 are separated by 114 nucleotides
11703 and 11704 are separated by 139 nucleotides
mRNA_3332: 11700 - K17424 MRPL43 large subunit ribosomal protein L43, at 411,009 to 411,517
11700
mRNA_3333: 11701 - K05283 PIGW phosphatidylinositol glycan, class W, at 411,670 to 413,695
11701
mRNA_3334: 11702 - K03936 NDUFS3 NADH dehydrogenase (ubiquinone) Fe-S protein 3, at 413,797 to 414,963
11702
mRNA_3335: 11703 - Hypothetical Protein, at 415,078 to 415,551
11703
mRNA_3336: 11704 - K07827 KRAS, KRAS2 GTPase KRas, at 415,691 to 416,703
11704
Group
Condition
mRNA
_3332
mRNA
_3333
mRNA
_3334
mRNA
_3335
mRNA
_3336
11700
11701
11702
11703
11704
buoyancy on lipid accumulation
40 hrs Median Buoyancy
N.D.
N.D.
N.D.
-0.3
-0.3
facs on lipid accumulation
Sorted Low BODIPY Gate
N.D.
N.D.
N.D.
-0.6
+0.0
growth on oleic acid
YNB Oleic Acid
N.D.
N.D.
N.D.
-0.1
-0.3
growth on ricinoleic acid
YNB Ricinoleic Acid
N.D.
N.D.
N.D.
-0.1
-0.2
buoyancy on lipid accumulation
40 hrs Low Buoyancy
N.D.
N.D.
N.D.
-0.1
-0.2
buoyancy on lipid accumulation
40 hrs High Buoyancy
N.D.
N.D.
N.D.
-0.3
+0.1
growth on oleic acid
YNB Glucose
N.D.
N.D.
N.D.
+0.0
-0.2
growth on ypd
YPD
N.D.
N.D.
N.D.
+0.1
-0.3
ypd buoyancy
40 hrs Low Buoyancy
N.D.
N.D.
N.D.
-0.1
-0.1
ypd buoyancy
40 hrs High Buoyancy
N.D.
N.D.
N.D.
-0.1
+0.0
facs on lipid accumulation
Sorted High BODIPY Gate
N.D.
N.D.
N.D.
-0.2
+0.2
auxotrophy
YNB Glucose 75mM Arginine
N.D.
N.D.
N.D.
+0.4
-0.4
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
N.D.
N.D.
N.D.
+0.1
-0.0
facs on lipid accumulation
Sorted No BODIPY Gate
N.D.
N.D.
N.D.
+0.5
-0.3
ypd buoyancy
40 hrs Median Buoyancy
N.D.
N.D.
N.D.
+0.3
-0.1
auxotrophy
YNB Glucose
N.D.
N.D.
N.D.
+0.6
-0.2
auxotrophy
YNB Glucose 75mM Methionine
N.D.
N.D.
N.D.
+0.4
-0.0
auxotrophy
Dropout Complete
N.D.
N.D.
N.D.
+0.6
-0.2
growth on ricinoleic acid
YNB Methylricinoleic Acid
N.D.
N.D.
N.D.
+0.3
+0.0
growth on ricinoleic acid
YNB Glucose
N.D.
N.D.
N.D.
+0.3
+0.1
ypd buoyancy
40 hrs No Buoyancy Separation
N.D.
N.D.
N.D.
+0.4
+0.1
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