Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11716 and 11717 are separated by 645 nucleotides11717 and 11718 are separated by 520 nucleotides11718 and 11719 are separated by 674 nucleotides11719 and 11720 are separated by 404 nucleotides mRNA_3348: 11716 - K09831 ERG5, CYP61A sterol 22-desaturase, at 447,247 to 449,563 11716 mRNA_3349: 11717 - K13179 DDX18, HAS1 ATP-dependent RNA helicase DDX18/HAS1, at 450,209 to 452,145 11717 mRNA_3350: 11718 - KOG1001 Helicase-like transcription factor HLTF/DNA helicase RAD5, DEAD-box superfamily, at 452,666 to 460,645 11718 mRNA_3351: 11719 - K12196 VPS4 vacuolar protein-sorting-associated protein 4, at 461,320 to 463,748 11719 mRNA_3352: 11720 - K06699 PSME4 proteasome activator subunit 4, at 464,153 to 471,579 11720
Group Condition mRNA_3348 mRNA_3349 mRNA_3350 mRNA_3351 mRNA_3352
11716 11717 11718 11719 11720
growth on ypd YPD N.D. N.D. -0.1 N.D. -0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -0.2 N.D. +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -0.2 N.D. +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.0 N.D. -0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.1 N.D. +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.2 N.D. +0.1
growth on oleic acid YNB Glucose N.D. N.D. -0.1 N.D. +0.1
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.1 N.D. +0.0
auxotrophy YNB Glucose N.D. N.D. -0.0 N.D. +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.1 N.D. +0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.0 N.D. +0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. +0.1 N.D. -0.0
auxotrophy Dropout Complete N.D. N.D. -0.1 N.D. +0.1
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +0.0 N.D. +0.1
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.0 N.D. +0.1
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.0 N.D. +0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +0.0 N.D. +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.0 N.D. +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.1 N.D. +0.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.1 N.D. +0.4
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.0 N.D. +0.9
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