Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11718 and 11719 are separated by 674 nucleotides11719 and 11720 are separated by 404 nucleotides11720 and 11721 are separated by 294 nucleotides11721 and 11722 are separated by 214 nucleotides mRNA_3350: 11718 - KOG1001 Helicase-like transcription factor HLTF/DNA helicase RAD5, DEAD-box superfamily, at 452,666 to 460,645 11718 mRNA_3351: 11719 - K12196 VPS4 vacuolar protein-sorting-associated protein 4, at 461,320 to 463,748 11719 mRNA_3352: 11720 - K06699 PSME4 proteasome activator subunit 4, at 464,153 to 471,579 11720 mRNA_3353: 11721 - K10352 MYH myosin heavy chain, at 471,874 to 478,185 11721 mRNA_3354: 11722 - KOG2395 Protein involved in vacuole import and degradation, at 478,400 to 482,104 11722
Group Condition mRNA_3350 mRNA_3351 mRNA_3352 mRNA_3353 mRNA_3354
11718 11719 11720 11721 11722
auxotrophy Dropout Complete -0.1 N.D. +0.1 N.D. -2.5
auxotrophy YNB Glucose -0.0 N.D. +0.0 N.D. -1.9
auxotrophy YNB Glucose 75mM Arginine -0.0 N.D. +0.1 N.D. -1.9
growth on ricinoleic acid YNB Glucose -0.1 N.D. +0.0 N.D. -1.6
growth on ypd YPD -0.1 N.D. -0.1 N.D. -1.5
auxotrophy YNB Glucose 75mM Methionine +0.0 N.D. +0.1 N.D. -1.5
facs on lipid accumulation Sorted No BODIPY Gate -0.0 N.D. +0.1 N.D. -1.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 N.D. +0.1 N.D. -1.0
growth on oleic acid YNB Glucose -0.1 N.D. +0.1 N.D. -0.8
growth on ricinoleic acid YNB Methylricinoleic Acid -0.0 N.D. +0.2 N.D. -0.8
facs on lipid accumulation Sorted High BODIPY Gate -0.1 N.D. +0.4 N.D. -0.8
ypd buoyancy 40 hrs High Buoyancy +0.0 N.D. +0.1 N.D. -0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 N.D. +0.0 N.D. +0.2
ypd buoyancy 40 hrs Low Buoyancy -0.0 N.D. -0.0 N.D. +0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 N.D. +0.1 N.D. +0.6
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. +0.0 N.D. +1.0
growth on oleic acid YNB Oleic Acid -0.0 N.D. +0.9 N.D. +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 N.D. +0.2 N.D. +1.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 N.D. +0.1 N.D. +1.8
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. +0.3 N.D. +1.7
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 N.D. -0.0 N.D. +2.4
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