Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11720 and 11721 are separated by 294 nucleotides11721 and 11722 are separated by 214 nucleotides11722 and 11723 are separated by 649 nucleotides11723 and 11724 are separated by 448 nucleotides mRNA_3352: 11720 - K06699 PSME4 proteasome activator subunit 4, at 464,153 to 471,579 11720 mRNA_3353: 11721 - K10352 MYH myosin heavy chain, at 471,874 to 478,185 11721 mRNA_3354: 11722 - KOG2395 Protein involved in vacuole import and degradation, at 478,400 to 482,104 11722 mRNA_3355: 11723 - K01126 E3.1.4.46, glpQ, ugpQ glycerophosphoryl diester phosphodiesterase, at 482,754 to 484,525 11723 mRNA_3356: 11724 - K19306 BUD23 18S rRNA (guanine1575-N7)-methyltransferase, at 484,974 to 486,354 11724
Group Condition mRNA_3352 mRNA_3353 mRNA_3354 mRNA_3355 mRNA_3356
11720 11721 11722 11723 11724
auxotrophy Dropout Complete +0.1 N.D. -2.5 +0.2 N.D.
growth on ypd YPD -0.1 N.D. -1.5 -0.2 N.D.
auxotrophy YNB Glucose +0.0 N.D. -1.9 +0.3 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.1 N.D. -1.9 +0.4 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. -1.5 +0.3 N.D.
growth on ricinoleic acid YNB Glucose +0.0 N.D. -1.6 +0.5 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. -1.2 +0.4 N.D.
growth on oleic acid YNB Glucose +0.1 N.D. -0.8 +0.1 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 N.D. -1.0 +0.4 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 N.D. -0.8 +0.4 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.4 N.D. -0.8 +0.4 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. -0.2 +0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.0 N.D. +0.6 -0.2 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 N.D. +0.2 +0.3 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 N.D. +0.6 +0.1 N.D.
growth on oleic acid YNB Oleic Acid +0.9 N.D. +0.2 -0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.0 N.D. +1.0 -0.0 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 N.D. +1.3 +0.2 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. +1.8 +0.1 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 N.D. +2.4 -0.4 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 N.D. +1.7 +0.1 N.D.
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