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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
11741 and 11742 are separated by 665 nucleotides
11742 and 11743 are separated by 537 nucleotides
11743 and 11744 are separated by 65 nucleotides
11744 and 11745 are separated by 134 nucleotides
mRNA_3373: 11741 - K00390 cysH phosphoadenosine phosphosulfate reductase, at 523,671 to 524,664
11741
mRNA_3374: 11742 - KOG2273 Membrane coat complex Retromer, subunit VPS5/SNX1, Sorting nexins, and related PX domain-containing proteins, at 525,330 to 527,850
11742
mRNA_3375: 11743 - Hypothetical Protein, at 528,388 to 528,831
11743
mRNA_3376: 11744 - K12864 CTNNBL1 beta-catenin-like protein 1, at 528,897 to 531,251
11744
mRNA_3377: 11745 - KOG1516 Carboxylesterase and related proteins, at 531,386 to 533,428
11745
Group
Condition
mRNA
_3373
mRNA
_3374
mRNA
_3375
mRNA
_3376
mRNA
_3377
11741
11742
11743
11744
11745
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.9
-1.3
-1.5
-0.5
-0.0
growth on ricinoleic acid
YNB Methylricinoleic Acid
-1.3
-0.1
-0.0
-0.2
-0.2
growth on ricinoleic acid
YNB Ricinoleic Acid
-1.0
-0.2
+0.0
-0.6
+0.0
growth on ricinoleic acid
YNB Glucose
-1.7
-0.1
+0.2
-0.1
+0.2
facs on lipid accumulation
Sorted No BODIPY Gate
-0.8
-0.4
-0.3
+0.0
-0.0
auxotrophy
YNB Glucose 75mM Arginine
-1.5
-0.4
+0.3
-0.0
+0.3
auxotrophy
YNB Glucose
-1.2
-0.1
+0.2
-0.1
+0.3
growth on oleic acid
YNB Glucose
-1.3
-0.1
+0.1
+0.2
+0.2
facs on lipid accumulation
Sorted High BODIPY Gate
+0.3
-0.8
+0.0
+0.1
-0.0
growth on oleic acid
YNB Oleic Acid
-0.4
+0.1
-0.1
-0.1
+0.3
ypd buoyancy
40 hrs Median Buoyancy
-0.2
-0.2
+0.2
-0.0
+0.1
ypd buoyancy
40 hrs No Buoyancy Separation
-0.6
+0.3
+0.1
-0.1
+0.2
buoyancy on lipid accumulation
40 hrs Low Buoyancy
-0.9
+0.2
+0.2
+0.3
+0.1
ypd buoyancy
40 hrs High Buoyancy
-0.2
+0.1
-0.1
-0.0
+0.2
growth on ypd
YPD
+0.2
-0.5
+0.4
-0.0
-0.1
auxotrophy
Dropout Complete
+0.0
+0.1
+0.0
-0.1
+0.1
ypd buoyancy
40 hrs Low Buoyancy
-0.1
-0.1
+0.1
+0.2
+0.1
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-0.7
+0.5
+0.3
+0.5
+0.2
auxotrophy
YNB Glucose 75mM Methionine
+0.1
-0.0
+0.5
+0.1
+0.3
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
+0.1
+0.5
+0.1
+0.2
+0.3
buoyancy on lipid accumulation
40 hrs High Buoyancy
+0.8
+0.2
-0.1
+0.4
+0.1
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