Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11751 and 11752 are separated by 1125 nucleotides11752 and 11753 are separated by 1016 nucleotides11753 and 11754 are separated by 348 nucleotides11754 and 11755 are separated by 105 nucleotides mRNA_3383: 11751 - HMMPfam-BSD domain-PF03909,ProSiteProfiles-BSD domain profile.-PS50858,SMART-domain in transcription factors and synapse-associated proteins-SM00751,SUPERFAMILY--SSF140383, at 548,745 to 549,848 11751 mRNA_3384: 11752 - K00963 UGP2, galU, galF UTP--glucose-1-phosphate uridylyltransferase, at 550,974 to 553,438 11752 mRNA_3385: 11753 - HMMPfam-Gamma interferon inducible lysosomal thiol reductase (GILT)-PF03227, at 554,455 to 555,770 11753 mRNA_3386: 11754 - K05756 ARPC3 actin related protein 2/3 complex, subunit 3, at 556,119 to 557,419 11754 mRNA_3387: 11755 - K01771 plc 1-phosphatidylinositol phosphodiesterase, at 557,525 to 558,870 11755
Group Condition mRNA_3383 mRNA_3384 mRNA_3385 mRNA_3386 mRNA_3387
11751 11752 11753 11754 11755
ypd buoyancy 40 hrs No Buoyancy Separation -4.2 N.D. -0.7 N.D. -0.1
growth on ricinoleic acid YNB Glucose -2.1 N.D. +0.1 N.D. -0.2
auxotrophy YNB Glucose 75mM Arginine -2.5 N.D. +0.3 N.D. +0.1
ypd buoyancy 40 hrs High Buoyancy -1.6 N.D. -0.2 N.D. -0.3
ypd buoyancy 40 hrs Low Buoyancy -1.5 N.D. -0.0 N.D. -0.3
growth on ricinoleic acid YNB Ricinoleic Acid -1.3 N.D. +0.3 N.D. +0.1
auxotrophy YNB Glucose 75mM Methionine -1.2 N.D. +0.2 N.D. -0.0
facs on lipid accumulation Sorted No BODIPY Gate -1.3 N.D. +0.1 N.D. +0.4
ypd buoyancy 40 hrs Median Buoyancy -0.4 N.D. -0.1 N.D. -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.8 N.D. +0.3 N.D. +0.1
growth on ypd YPD +0.5 N.D. -0.3 N.D. -0.3
growth on oleic acid YNB Glucose +0.1 N.D. -0.0 N.D. -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 N.D. +0.1 N.D. +0.1
auxotrophy Dropout Complete -0.2 N.D. +0.2 N.D. +0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 N.D. +0.3 N.D. -0.3
growth on oleic acid YNB Oleic Acid +1.1 N.D. +0.0 N.D. -0.0
auxotrophy YNB Glucose +1.0 N.D. +0.4 N.D. -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +1.7 N.D. +0.2 N.D. +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +2.8 N.D. +0.2 N.D. +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +2.8 N.D. +0.3 N.D. +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +3.4 N.D. +0.4 N.D. +0.3
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