Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11752 and 11753 are separated by 1016 nucleotides11753 and 11754 are separated by 348 nucleotides11754 and 11755 are separated by 105 nucleotides11755 and 11756 are separated by 650 nucleotides mRNA_3384: 11752 - K00963 UGP2, galU, galF UTP--glucose-1-phosphate uridylyltransferase, at 550,974 to 553,438 11752 mRNA_3385: 11753 - HMMPfam-Gamma interferon inducible lysosomal thiol reductase (GILT)-PF03227, at 554,455 to 555,770 11753 mRNA_3386: 11754 - K05756 ARPC3 actin related protein 2/3 complex, subunit 3, at 556,119 to 557,419 11754 mRNA_3387: 11755 - K01771 plc 1-phosphatidylinositol phosphodiesterase, at 557,525 to 558,870 11755 mRNA_3388: 11756 - KOG1577 Aldo/keto reductase family proteins, at 559,521 to 561,050 11756
Group Condition mRNA_3384 mRNA_3385 mRNA_3386 mRNA_3387 mRNA_3388
11752 11753 11754 11755 11756
growth on ypd YPD N.D. -0.3 N.D. -0.3 -0.4
ypd buoyancy 40 hrs High Buoyancy N.D. -0.2 N.D. -0.3 +0.0
growth on oleic acid YNB Glucose N.D. -0.0 N.D. -0.2 -0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.0 N.D. -0.3 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.7 N.D. -0.1 +0.6
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 N.D. -0.1 +0.1
auxotrophy Dropout Complete N.D. +0.2 N.D. +0.1 -0.3
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.3 N.D. -0.3 -0.0
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.3 N.D. +0.1 -0.4
auxotrophy YNB Glucose 75mM Methionine N.D. +0.2 N.D. -0.0 -0.1
growth on ricinoleic acid YNB Glucose N.D. +0.1 N.D. -0.2 +0.3
auxotrophy YNB Glucose N.D. +0.4 N.D. -0.0 -0.1
auxotrophy YNB Glucose 75mM Arginine N.D. +0.3 N.D. +0.1 -0.1
growth on oleic acid YNB Oleic Acid N.D. +0.0 N.D. -0.0 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.3 N.D. +0.1 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.1 N.D. +0.1 +0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.1 N.D. +0.4 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.2 N.D. +0.2 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.3 N.D. +0.2 +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.4 N.D. +0.3 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.2 N.D. +0.1 +0.7
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