Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11762 and 11763 are separated by 157 nucleotides11763 and 11764 are separated by 562 nucleotides11764 and 11765 are separated by 325 nucleotides11765 and 11766 are separated by 396 nucleotides mRNA_3394: 11762 - HMMPfam-UreF-PF01730, at 574,156 to 575,292 11762 mRNA_3395: 11763 - K07566 tsaC, rimN, SUA5 L-threonylcarbamoyladenylate synthase, at 575,450 to 577,006 11763 mRNA_3396: 11764 - HMMPfam-Metallo-beta-lactamase superfamily-PF00753,SMART-Metallo-beta-lactamase superfamily-SM00849,SUPERFAMILY--SSF56281, at 577,569 to 578,953 11764 mRNA_3397: 11765 - K13370 HSD17B8 17beta-estradiol 17-dehydrogenase / 3alpha(17beta)-hydroxysteroid dehydrogenase (NAD+), at 579,279 to 580,457 11765 mRNA_3398: 11766 - Hypothetical Protein, at 580,854 to 581,831 11766
Group Condition mRNA_3394 mRNA_3395 mRNA_3396 mRNA_3397 mRNA_3398
11762 11763 11764 11765 11766
growth on ricinoleic acid YNB Glucose -0.2 N.D. +0.1 -0.2 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 N.D. -0.2 +0.4 -0.8
auxotrophy YNB Glucose +0.0 N.D. -0.0 -0.1 +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.2 N.D. +0.1 -0.2 -0.1
auxotrophy Dropout Complete -0.2 N.D. +0.1 -0.0 +0.3
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. +0.0 -0.2 +0.2
ypd buoyancy 40 hrs High Buoyancy +0.2 N.D. +0.1 -0.1 -0.0
auxotrophy YNB Glucose 75mM Methionine -0.1 N.D. +0.2 -0.0 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 N.D. +0.2 +0.1 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.5 N.D. +0.3 -0.4 +0.0
auxotrophy YNB Glucose 75mM Arginine -0.1 N.D. +0.2 +0.0 +0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.5 N.D. +0.5 +0.5 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.5 N.D. +0.1 -0.0 -0.0
growth on oleic acid YNB Glucose +0.1 N.D. +0.3 +0.0 +0.1
growth on oleic acid YNB Oleic Acid +0.1 N.D. +0.1 +0.0 +0.4
facs on lipid accumulation Sorted No BODIPY Gate -0.2 N.D. +0.3 +0.3 +0.4
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 N.D. +0.4 +0.1 +0.7
growth on ypd YPD +0.1 N.D. +0.2 +0.4 +0.3
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 N.D. +0.5 -0.1 +1.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.7 N.D. -0.0 +0.3 +0.7
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.5 N.D. +0.1 +0.3 +0.8
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