Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11786 and 11787 are separated by 1100 nucleotides11787 and 11788 are separated by 390 nucleotides11788 and 11789 are separated by 443 nucleotides11789 and 11790 are separated by 119 nucleotides mRNA_3418: 11786 - KOG1502 Flavonol reductase/cinnamoyl-CoA reductase, at 47,383 to 48,909 11786 mRNA_3419: 11787 - K01120 cpdP 3',5'-cyclic-nucleotide phosphodiesterase, at 50,010 to 53,314 11787 mRNA_3420: 11788 - HMMPfam-ATP dependent DNA ligase domain-PF01068,HMMPfam-DNA ligase N terminus-PF04675,ProSiteProfiles-ATP-dependent DNA ligase family profile.-PS50160,SUPERFAMILY--SSF56091, at 53,705 to 57,912 11788 mRNA_3421: 11789 - K14297 NUP98, ADAR2, NUP116 nuclear pore complex protein Nup98-Nup96, at 58,356 to 61,690 11789 mRNA_3422: 11790 - KOG2882 p-Nitrophenyl phosphatase, at 61,810 to 63,146 11790
Group Condition mRNA_3418 mRNA_3419 mRNA_3420 mRNA_3421 mRNA_3422
11786 11787 11788 11789 11790
facs on lipid accumulation Sorted High BODIPY Gate -1.4 -1.4 -0.3 N.D. -0.4
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 -2.2 -0.4 N.D. +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 -1.3 -0.3 N.D. -0.2
ypd buoyancy 40 hrs Low Buoyancy -0.6 -1.3 +0.1 N.D. +0.4
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -0.9 -0.3 N.D. -0.0
growth on ypd YPD -0.9 -0.3 +0.0 N.D. -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.9 +0.0 -0.1 N.D. -0.3
ypd buoyancy 40 hrs High Buoyancy -1.8 +0.1 +0.4 N.D. +0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 -0.6 -0.4 N.D. +0.1
ypd buoyancy 40 hrs No Buoyancy Separation -1.5 +0.1 +0.3 N.D. +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 -0.5 +0.0 N.D. -0.1
auxotrophy YNB Glucose 75mM Arginine -0.4 -0.3 +0.4 N.D. -0.4
growth on ricinoleic acid YNB Glucose +0.3 -0.7 -0.3 N.D. +0.0
growth on oleic acid YNB Oleic Acid +0.7 -0.7 +0.4 N.D. -1.0
growth on oleic acid YNB Glucose -0.4 -0.1 -0.0 N.D. -0.0
auxotrophy Dropout Complete -0.2 -0.2 +0.5 N.D. -0.4
auxotrophy YNB Glucose 75mM Methionine +0.0 -0.4 +0.3 N.D. -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 -0.3 +0.0 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 +0.4 -0.1 N.D. -0.1
auxotrophy YNB Glucose +0.5 -0.2 +0.1 N.D. -0.5
ypd buoyancy 40 hrs Median Buoyancy +0.5 -0.0 +0.3 N.D. +0.2
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