Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11799 and 11800 are separated by 1217 nucleotides11800 and 11801 are separated by 871 nucleotides11801 and 11802 are separated by 288 nucleotides11802 and 11803 are separated by 473 nucleotides mRNA_3431: 11799 - K11155 DGAT1 diacylglycerol O-acyltransferase 1, at 82,671 to 85,458 11799 mRNA_3432: 11800 - BLAST RecF/RecN/SMC protein [Sistotremastrum niveocremeum H..., at 86,676 to 87,359 11800 mRNA_3433: 11801 - HMMPfam-Rtr1/RPAP2 family-PF04181,HMMPfam-VMA21-like domain-PF09446,ProSiteProfiles-RTR1-type zinc finger.-PS51479, at 88,231 to 90,113 11801 mRNA_3434: 11802 - K00237 SDHD, SDH4 succinate dehydrogenase (ubiquinone) membrane anchor subunit, at 90,402 to 91,512 11802 mRNA_3435: 11803 - K12614 DDX6, RCK, DHH1 ATP-dependent RNA helicase DDX6/DHH1, at 91,986 to 94,017 11803
Group Condition mRNA_3431 mRNA_3432 mRNA_3433 mRNA_3434 mRNA_3435
11799 11800 11801 11802 11803
ypd buoyancy 40 hrs No Buoyancy Separation -0.5 -0.2 -0.0 N.D. -0.0
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 +0.1 +0.3 N.D. -0.8
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 -0.2 +0.1 N.D. -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 -0.0 +0.0 N.D. -0.9
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 -0.0 +0.0 N.D. -0.8
growth on ricinoleic acid YNB Glucose +0.1 -0.1 +0.1 N.D. -0.5
auxotrophy YNB Glucose 75mM Arginine +0.4 -0.3 -0.0 N.D. -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 -0.1 -0.0 N.D. -0.5
auxotrophy Dropout Complete +0.4 -0.4 +0.0 N.D. -0.3
growth on ypd YPD +0.3 -0.1 +0.0 N.D. -0.5
ypd buoyancy 40 hrs High Buoyancy -0.2 +0.2 +0.0 N.D. -0.1
growth on oleic acid YNB Glucose +0.3 +0.1 -0.1 N.D. -0.5
auxotrophy YNB Glucose +0.4 -0.2 +0.0 N.D. -0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.4 +0.0 -0.1 N.D. -0.3
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.1 -0.0 N.D. +0.2
auxotrophy YNB Glucose 75mM Methionine +0.5 +0.0 +0.0 N.D. -0.4
growth on oleic acid YNB Oleic Acid +0.0 +0.1 +0.3 N.D. -0.2
ypd buoyancy 40 hrs Low Buoyancy +0.4 -0.1 +0.2 N.D. -0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.0 +0.3 -0.3 N.D. +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +0.1 +0.3 N.D. -0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.4 -0.0 +0.0 N.D. -0.0
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