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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
11801 and 11802 are separated by 288 nucleotides
11802 and 11803 are separated by 473 nucleotides
11803 and 11804 are separated by 389 nucleotides
11804 and 11805 are separated by 147 nucleotides
mRNA_3433: 11801 - HMMPfam-Rtr1/RPAP2 family-PF04181,HMMPfam-VMA21-like domain-PF09446,ProSiteProfiles-RTR1-type zinc finger.-PS51479, at 88,231 to 90,113
11801
mRNA_3434: 11802 - K00237 SDHD, SDH4 succinate dehydrogenase (ubiquinone) membrane anchor subunit, at 90,402 to 91,512
11802
mRNA_3435: 11803 - K12614 DDX6, RCK, DHH1 ATP-dependent RNA helicase DDX6/DHH1, at 91,986 to 94,017
11803
mRNA_3436: 11804 - HMMPfam-Oligosaccharyltransferase subunit Ribophorin II-PF05817, at 94,407 to 97,285
11804
mRNA_3437: 11805 - K03033 PSMD3, RPN3 26S proteasome regulatory subunit N3, at 97,433 to 99,630
11805
Group
Condition
mRNA
_3433
mRNA
_3434
mRNA
_3435
mRNA
_3436
mRNA
_3437
11801
11802
11803
11804
11805
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
+0.0
N.D.
-0.9
-0.3
+0.4
buoyancy on lipid accumulation
40 hrs High Buoyancy
+0.0
N.D.
-0.8
-0.0
+0.2
facs on lipid accumulation
Sorted Low BODIPY Gate
+0.1
N.D.
-0.4
-0.3
+0.3
growth on ypd
YPD
+0.0
N.D.
-0.5
-0.1
+0.3
buoyancy on lipid accumulation
40 hrs Low Buoyancy
-0.0
N.D.
-0.5
+0.0
+0.3
growth on ricinoleic acid
YNB Ricinoleic Acid
+0.3
N.D.
-0.8
-0.3
+0.6
growth on ricinoleic acid
YNB Glucose
+0.1
N.D.
-0.5
-0.2
+0.4
growth on oleic acid
YNB Glucose
-0.1
N.D.
-0.5
-0.1
+0.5
ypd buoyancy
40 hrs No Buoyancy Separation
-0.0
N.D.
-0.0
+0.3
-0.3
ypd buoyancy
40 hrs High Buoyancy
+0.0
N.D.
-0.1
-0.0
+0.0
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-0.1
N.D.
-0.3
+0.0
+0.3
facs on lipid accumulation
Sorted High BODIPY Gate
-0.3
N.D.
+0.3
-0.2
+0.3
auxotrophy
YNB Glucose
+0.0
N.D.
-0.4
-0.1
+0.6
auxotrophy
YNB Glucose 75mM Methionine
+0.0
N.D.
-0.4
+0.0
+0.6
auxotrophy
YNB Glucose 75mM Arginine
-0.0
N.D.
-0.4
-0.0
+0.6
ypd buoyancy
40 hrs Median Buoyancy
-0.0
N.D.
+0.2
+0.0
+0.0
auxotrophy
Dropout Complete
+0.0
N.D.
-0.3
-0.1
+0.7
growth on ricinoleic acid
YNB Methylricinoleic Acid
+0.3
N.D.
-0.2
-0.3
+0.6
ypd buoyancy
40 hrs Low Buoyancy
+0.2
N.D.
-0.2
+0.1
+0.5
growth on oleic acid
YNB Oleic Acid
+0.3
N.D.
-0.2
-0.1
+0.7
facs on lipid accumulation
Sorted No BODIPY Gate
+0.0
N.D.
-0.0
+0.3
+0.5
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