Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11801 and 11802 are separated by 288 nucleotides11802 and 11803 are separated by 473 nucleotides11803 and 11804 are separated by 389 nucleotides11804 and 11805 are separated by 147 nucleotides mRNA_3433: 11801 - HMMPfam-Rtr1/RPAP2 family-PF04181,HMMPfam-VMA21-like domain-PF09446,ProSiteProfiles-RTR1-type zinc finger.-PS51479, at 88,231 to 90,113 11801 mRNA_3434: 11802 - K00237 SDHD, SDH4 succinate dehydrogenase (ubiquinone) membrane anchor subunit, at 90,402 to 91,512 11802 mRNA_3435: 11803 - K12614 DDX6, RCK, DHH1 ATP-dependent RNA helicase DDX6/DHH1, at 91,986 to 94,017 11803 mRNA_3436: 11804 - HMMPfam-Oligosaccharyltransferase subunit Ribophorin II-PF05817, at 94,407 to 97,285 11804 mRNA_3437: 11805 - K03033 PSMD3, RPN3 26S proteasome regulatory subunit N3, at 97,433 to 99,630 11805
Group Condition mRNA_3433 mRNA_3434 mRNA_3435 mRNA_3436 mRNA_3437
11801 11802 11803 11804 11805
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 N.D. -0.9 -0.3 +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 N.D. -0.8 -0.0 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 N.D. -0.4 -0.3 +0.3
growth on ypd YPD +0.0 N.D. -0.5 -0.1 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 N.D. -0.5 +0.0 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 N.D. -0.8 -0.3 +0.6
growth on ricinoleic acid YNB Glucose +0.1 N.D. -0.5 -0.2 +0.4
growth on oleic acid YNB Glucose -0.1 N.D. -0.5 -0.1 +0.5
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 N.D. -0.0 +0.3 -0.3
ypd buoyancy 40 hrs High Buoyancy +0.0 N.D. -0.1 -0.0 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 N.D. -0.3 +0.0 +0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.3 N.D. +0.3 -0.2 +0.3
auxotrophy YNB Glucose +0.0 N.D. -0.4 -0.1 +0.6
auxotrophy YNB Glucose 75mM Methionine +0.0 N.D. -0.4 +0.0 +0.6
auxotrophy YNB Glucose 75mM Arginine -0.0 N.D. -0.4 -0.0 +0.6
ypd buoyancy 40 hrs Median Buoyancy -0.0 N.D. +0.2 +0.0 +0.0
auxotrophy Dropout Complete +0.0 N.D. -0.3 -0.1 +0.7
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 N.D. -0.2 -0.3 +0.6
ypd buoyancy 40 hrs Low Buoyancy +0.2 N.D. -0.2 +0.1 +0.5
growth on oleic acid YNB Oleic Acid +0.3 N.D. -0.2 -0.1 +0.7
facs on lipid accumulation Sorted No BODIPY Gate +0.0 N.D. -0.0 +0.3 +0.5
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