Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11811 and 11812 are separated by 222 nucleotides11812 and 11813 are separated by 843 nucleotides11813 and 11814 are separated by 653 nucleotides11814 and 11815 are separated by 377 nucleotides mRNA_3443: 11811 - K06694 PSMD10 26S proteasome non-ATPase regulatory subunit 10, at 116,448 to 118,253 11811 mRNA_3444: 11812 - Hypothetical Protein, at 118,476 to 119,519 11812 mRNA_3445: 11813 - HMMPfam-BRO1-like domain-PF03097,ProSiteProfiles-BRO1 domain profile.-PS51180,ProSiteProfiles-Prokaryotic membrane lipoprotein lipid attachment site profile.-PS51257,SMART-BRO1-like domain-SM01041, at 120,363 to 121,884 11813 mRNA_3446: 11814 - K07393 ECM4 putative glutathione S-transferase, at 122,538 to 123,846 11814 mRNA_3447: 11815 - HMMPfam-AhpC/TSA antioxidant enzyme-PF13911,SUPERFAMILY--SSF52833, at 124,224 to 125,009 11815
Group Condition mRNA_3443 mRNA_3444 mRNA_3445 mRNA_3446 mRNA_3447
11811 11812 11813 11814 11815
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 -0.2 -0.0 -0.3 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 +0.1 -0.8 +0.2 N.D.
growth on ypd YPD -0.3 -0.2 +0.1 +0.0 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.4 +0.2 -0.7 -0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.2 -0.5 +0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.2 -0.1 -0.1 -0.0 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.5 +0.3 +0.3 -0.2 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.5 +0.3 -0.7 -0.1 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 +0.4 -0.3 +0.3 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.0 +0.3 +0.1 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 +0.2 -0.0 +0.2 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.0 +0.4 -0.3 +0.3 N.D.
growth on oleic acid YNB Glucose +0.0 -0.1 +0.6 -0.1 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 +0.3 -0.3 +0.5 N.D.
growth on oleic acid YNB Oleic Acid +0.2 +0.0 +0.3 +0.3 N.D.
auxotrophy Dropout Complete +0.3 +0.0 +0.7 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.7 -0.3 +0.5 +0.1 N.D.
growth on ricinoleic acid YNB Glucose -0.1 +0.5 +0.7 +0.1 N.D.
auxotrophy YNB Glucose +0.4 -0.0 +0.8 +0.3 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.2 -0.1 +0.8 +0.6 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.3 +0.0 +0.6 +0.6 N.D.
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