Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11816 and 11817 are separated by 82 nucleotides11817 and 11818 are separated by 762 nucleotides11818 and 11819 are separated by 622 nucleotides11819 and 11820 are separated by 85 nucleotides mRNA_3448: 11816 - HMMPfam-Peptidase M50B-like-PF13398, at 125,268 to 126,548 11816 mRNA_3449: 11817 - HMMPfam-Integral peroxisomal membrane peroxin-PF06398, at 126,631 to 128,651 11817 mRNA_3450: 11818 - K20131 RABGEF1 Rab5 GDP/GTP exchange factor, at 129,414 to 132,360 11818 mRNA_3451: 11819 - HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 132,983 to 136,040 11819 mRNA_3452: 11820 - HMMPfam-Protein of unknown function (DUF2454)-PF10521,SUPERFAMILY--SSF48371, at 136,126 to 138,036 11820
Group Condition mRNA_3448 mRNA_3449 mRNA_3450 mRNA_3451 mRNA_3452
11816 11817 11818 11819 11820
ypd buoyancy 40 hrs Median Buoyancy -0.6 N.D. -0.1 -0.1 N.D.
auxotrophy YNB Glucose +0.3 N.D. -0.4 -0.4 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.4 N.D. -0.2 +0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.2 N.D. -0.2 +0.1 N.D.
auxotrophy Dropout Complete -0.0 N.D. +0.1 -0.5 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 N.D. -0.3 +0.2 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.0 N.D. -0.3 -0.0 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.2 N.D. +0.0 -0.1 N.D.
growth on ricinoleic acid YNB Glucose +0.2 N.D. +0.1 -0.3 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.2 N.D. +0.2 -0.2 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 N.D. -0.4 +0.4 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. +0.1 -0.0 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 N.D. -0.2 +0.2 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 N.D. -0.1 -0.1 N.D.
growth on oleic acid YNB Glucose +0.1 N.D. +0.2 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 N.D. -0.2 +0.3 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 N.D. +0.0 +0.4 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 N.D. +0.1 +0.1 N.D.
growth on ypd YPD +0.3 N.D. +0.2 +0.2 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.0 N.D. +0.3 +0.4 N.D.
growth on oleic acid YNB Oleic Acid +0.5 N.D. +0.2 +0.1 N.D.
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