Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11817 and 11818 are separated by 762 nucleotides11818 and 11819 are separated by 622 nucleotides11819 and 11820 are separated by 85 nucleotides11820 and 11821 are separated by 313 nucleotides mRNA_3449: 11817 - HMMPfam-Integral peroxisomal membrane peroxin-PF06398, at 126,631 to 128,651 11817 mRNA_3450: 11818 - K20131 RABGEF1 Rab5 GDP/GTP exchange factor, at 129,414 to 132,360 11818 mRNA_3451: 11819 - HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 132,983 to 136,040 11819 mRNA_3452: 11820 - HMMPfam-Protein of unknown function (DUF2454)-PF10521,SUPERFAMILY--SSF48371, at 136,126 to 138,036 11820 mRNA_3453: 11821 - KOG2959 Transcriptional regulator, at 138,350 to 139,548 11821
Group Condition mRNA_3449 mRNA_3450 mRNA_3451 mRNA_3452 mRNA_3453
11817 11818 11819 11820 11821
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.3 -0.0 N.D. -1.0
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.4 +0.4 N.D. -1.1
auxotrophy YNB Glucose N.D. -0.4 -0.4 N.D. -0.1
growth on ypd YPD N.D. +0.2 +0.2 N.D. -1.4
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.2 +0.2 N.D. -0.9
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.3 +0.2 N.D. -0.7
growth on ricinoleic acid YNB Glucose N.D. +0.1 -0.3 N.D. -0.5
auxotrophy Dropout Complete N.D. +0.1 -0.5 N.D. -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.1 -0.1 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.2 +0.3 N.D. -0.4
auxotrophy YNB Glucose 75mM Arginine N.D. +0.2 -0.2 N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy N.D. -0.2 +0.1 N.D. +0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 -0.1 N.D. +0.0
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.1 +0.1 N.D. -0.2
auxotrophy YNB Glucose 75mM Methionine N.D. +0.1 -0.0 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.2 +0.1 N.D. +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.0 +0.4 N.D. -0.4
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.0 -0.1 N.D. +0.4
growth on oleic acid YNB Oleic Acid N.D. +0.2 +0.1 N.D. +0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.3 +0.4 N.D. -0.3
growth on oleic acid YNB Glucose N.D. +0.2 -0.1 N.D. +0.5
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