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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
11819 and 11820 are separated by 85 nucleotides
11820 and 11821 are separated by 313 nucleotides
11821 and 11822 are separated by 96 nucleotides
11822 and 11823 are separated by 93 nucleotides
mRNA_3451: 11819 - HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 132,983 to 136,040
11819
mRNA_3452: 11820 - HMMPfam-Protein of unknown function (DUF2454)-PF10521,SUPERFAMILY--SSF48371, at 136,126 to 138,036
11820
mRNA_3453: 11821 - KOG2959 Transcriptional regulator, at 138,350 to 139,548
11821
mRNA_3454: 11822 - SUPERFAMILY--SSF52047, at 139,645 to 140,934
11822
mRNA_3455: 11823 - K03424 tatD TatD DNase family protein, at 141,028 to 142,309
11823
Group
Condition
mRNA
_3451
mRNA
_3452
mRNA
_3453
mRNA
_3454
mRNA
_3455
11819
11820
11821
11822
11823
buoyancy on lipid accumulation
40 hrs High Buoyancy
+0.2
N.D.
-0.9
-0.2
-0.3
auxotrophy
Dropout Complete
-0.5
N.D.
-0.3
-0.2
-0.2
growth on ypd
YPD
+0.2
N.D.
-1.4
+0.2
-0.1
growth on ricinoleic acid
YNB Glucose
-0.3
N.D.
-0.5
+0.2
-0.3
facs on lipid accumulation
Sorted No BODIPY Gate
-0.0
N.D.
-1.0
+0.5
-0.4
auxotrophy
YNB Glucose 75mM Arginine
-0.2
N.D.
-0.1
+0.0
-0.4
buoyancy on lipid accumulation
40 hrs Median Buoyancy
+0.3
N.D.
-0.4
-0.1
-0.4
auxotrophy
YNB Glucose
-0.4
N.D.
-0.1
+0.0
-0.1
auxotrophy
YNB Glucose 75mM Methionine
-0.0
N.D.
-0.1
-0.1
-0.2
ypd buoyancy
40 hrs No Buoyancy Separation
+0.4
N.D.
-1.1
+0.2
+0.1
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
+0.4
N.D.
-0.4
-0.1
-0.3
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.1
N.D.
-0.1
+0.2
-0.2
growth on ricinoleic acid
YNB Ricinoleic Acid
+0.1
N.D.
-0.2
+0.2
-0.4
facs on lipid accumulation
Sorted Low BODIPY Gate
+0.2
N.D.
-0.7
-0.1
+0.5
ypd buoyancy
40 hrs Median Buoyancy
-0.1
N.D.
+0.0
+0.1
-0.0
ypd buoyancy
40 hrs High Buoyancy
+0.1
N.D.
+0.0
+0.1
-0.2
facs on lipid accumulation
Sorted High BODIPY Gate
+0.4
N.D.
-0.3
+0.3
-0.4
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.1
N.D.
+0.2
+0.2
-0.2
growth on oleic acid
YNB Oleic Acid
+0.1
N.D.
+0.1
+0.1
+0.0
ypd buoyancy
40 hrs Low Buoyancy
-0.1
N.D.
+0.4
+0.3
+0.0
growth on oleic acid
YNB Glucose
-0.1
N.D.
+0.5
+0.0
+0.1
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