Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11819 and 11820 are separated by 85 nucleotides11820 and 11821 are separated by 313 nucleotides11821 and 11822 are separated by 96 nucleotides11822 and 11823 are separated by 93 nucleotides mRNA_3451: 11819 - HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 132,983 to 136,040 11819 mRNA_3452: 11820 - HMMPfam-Protein of unknown function (DUF2454)-PF10521,SUPERFAMILY--SSF48371, at 136,126 to 138,036 11820 mRNA_3453: 11821 - KOG2959 Transcriptional regulator, at 138,350 to 139,548 11821 mRNA_3454: 11822 - SUPERFAMILY--SSF52047, at 139,645 to 140,934 11822 mRNA_3455: 11823 - K03424 tatD TatD DNase family protein, at 141,028 to 142,309 11823
Group Condition mRNA_3451 mRNA_3452 mRNA_3453 mRNA_3454 mRNA_3455
11819 11820 11821 11822 11823
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 N.D. -0.9 -0.2 -0.3
auxotrophy Dropout Complete -0.5 N.D. -0.3 -0.2 -0.2
growth on ypd YPD +0.2 N.D. -1.4 +0.2 -0.1
growth on ricinoleic acid YNB Glucose -0.3 N.D. -0.5 +0.2 -0.3
facs on lipid accumulation Sorted No BODIPY Gate -0.0 N.D. -1.0 +0.5 -0.4
auxotrophy YNB Glucose 75mM Arginine -0.2 N.D. -0.1 +0.0 -0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 N.D. -0.4 -0.1 -0.4
auxotrophy YNB Glucose -0.4 N.D. -0.1 +0.0 -0.1
auxotrophy YNB Glucose 75mM Methionine -0.0 N.D. -0.1 -0.1 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.4 N.D. -1.1 +0.2 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.4 N.D. -0.4 -0.1 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 N.D. -0.1 +0.2 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 N.D. -0.2 +0.2 -0.4
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 N.D. -0.7 -0.1 +0.5
ypd buoyancy 40 hrs Median Buoyancy -0.1 N.D. +0.0 +0.1 -0.0
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. +0.0 +0.1 -0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.4 N.D. -0.3 +0.3 -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 N.D. +0.2 +0.2 -0.2
growth on oleic acid YNB Oleic Acid +0.1 N.D. +0.1 +0.1 +0.0
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. +0.4 +0.3 +0.0
growth on oleic acid YNB Glucose -0.1 N.D. +0.5 +0.0 +0.1
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