Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11859 and 11860 are separated by 212 nucleotides11860 and 11861 are separated by 429 nucleotides11861 and 11862 are separated by 638 nucleotides11862 and 11863 are separated by 264 nucleotides mRNA_3491: 11859 - K03007 RPB10, POLR2L DNA-directed RNA polymerases I, II, and III subunit RPABC5, at 232,549 to 233,056 11859 mRNA_3492: 11860 - HMMPfam-Cytidine and deoxycytidylate deaminase zinc-binding region-PF00383,SUPERFAMILY--SSF53927, at 233,269 to 234,150 11860 mRNA_3493: 11861 - K06944 K06944 uncharacterized protein, at 234,580 to 236,697 11861 mRNA_3494: 11862 - SUPERFAMILY--SSF160104, at 237,336 to 238,428 11862 mRNA_3495: 11863 - KOG4118 Uncharacterized conserved protein, at 238,693 to 239,237 11863
Group Condition mRNA_3491 mRNA_3492 mRNA_3493 mRNA_3494 mRNA_3495
11859 11860 11861 11862 11863
auxotrophy YNB Glucose 75mM Methionine N.D. -0.2 -0.6 -0.3 N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.8 -0.3 -0.0 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -1.1 -0.1 +0.1 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.2 -0.6 -0.4 N.D.
auxotrophy Dropout Complete N.D. -0.5 -0.6 +0.3 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.4 -0.2 -0.2 N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.1 -0.5 +0.0 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.1 -0.1 -0.6 N.D.
auxotrophy YNB Glucose N.D. +0.0 -0.5 +0.2 N.D.
growth on ricinoleic acid YNB Glucose N.D. +0.1 -0.3 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.2 -0.2 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.6 +0.0 -0.5 N.D.
growth on oleic acid YNB Glucose N.D. +0.1 -0.4 +0.5 N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. +0.4 -0.3 +0.1 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.3 +0.9 -0.4 N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.2 +0.3 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.4 -0.2 +0.2 N.D.
growth on oleic acid YNB Oleic Acid N.D. +0.4 -0.3 +0.4 N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.4 +0.2 -0.1 N.D.
growth on ypd YPD N.D. +0.8 -0.1 +0.3 N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. +0.5 +0.7 +0.1 N.D.
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