Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11869 and 11870 are separated by 46 nucleotides11870 and 11871 are separated by 404 nucleotides11871 and 11872 are separated by 1176 nucleotides11872 and 11873 are separated by 329 nucleotides mRNA_3501: 11869 - KOG3791 Predicted RNA-binding protein involved in translational regulation, at 248,480 to 250,835 11869 mRNA_3502: 11870 - Hypothetical Protein, at 250,882 to 251,460 11870 mRNA_3503: 11871 - K03457 TC.NCS1 nucleobase-cation symporter-1, NCS1 family, at 251,865 to 254,204 11871 mRNA_3504: 11872 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 255,381 to 257,476 11872 mRNA_3505: 11873 - KOG3699 Cytoskeletal protein Adducin, at 257,806 to 259,201 11873
Group Condition mRNA_3501 mRNA_3502 mRNA_3503 mRNA_3504 mRNA_3505
11869 11870 11871 11872 11873
growth on oleic acid YNB Oleic Acid -0.7 N.D. +0.3 +0.2 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 N.D. +0.1 -0.2 +0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.2 N.D. +0.0 -0.2 +0.1
auxotrophy YNB Glucose -1.3 N.D. +0.3 +0.5 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 N.D. -0.2 +0.4 -0.3
facs on lipid accumulation Sorted High BODIPY Gate +0.0 N.D. -0.0 -0.1 +0.1
growth on oleic acid YNB Glucose -0.5 N.D. +0.4 +0.1 -0.0
auxotrophy Dropout Complete -0.5 N.D. +0.2 +0.2 +0.1
growth on ypd YPD +0.7 N.D. -0.5 +0.1 -0.2
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. -0.0 -0.0 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 N.D. -0.1 +0.3 +0.4
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 N.D. +0.0 +0.3 +0.4
ypd buoyancy 40 hrs No Buoyancy Separation +1.0 N.D. -0.2 -0.2 -0.1
growth on ricinoleic acid YNB Glucose -0.0 N.D. +0.1 +0.0 +0.5
ypd buoyancy 40 hrs High Buoyancy +0.3 N.D. -0.1 +0.1 +0.3
auxotrophy YNB Glucose 75mM Methionine -0.4 N.D. +0.2 +0.4 +0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 N.D. -0.1 +0.3 +0.5
auxotrophy YNB Glucose 75mM Arginine -0.2 N.D. +0.3 +0.6 +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.5 N.D. +0.0 +0.0 +0.5
growth on ricinoleic acid YNB Ricinoleic Acid +0.6 N.D. +0.2 +0.1 +0.3
ypd buoyancy 40 hrs Low Buoyancy +0.6 N.D. +0.0 +0.2 +0.4
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