Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11871 and 11872 are separated by 1176 nucleotides11872 and 11873 are separated by 329 nucleotides11873 and 11874 are separated by 432 nucleotides11874 and 11875 are separated by 214 nucleotides mRNA_3503: 11871 - K03457 TC.NCS1 nucleobase-cation symporter-1, NCS1 family, at 251,865 to 254,204 11871 mRNA_3504: 11872 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 255,381 to 257,476 11872 mRNA_3505: 11873 - KOG3699 Cytoskeletal protein Adducin, at 257,806 to 259,201 11873 mRNA_3506: 11874 - HMMPfam-Asp/Glu/Hydantoin racemase-PF01177, at 259,634 to 260,419 11874 mRNA_3507: 11875 - K12251 aguB N-carbamoylputrescine amidase, at 260,634 to 261,961 11875
Group Condition mRNA_3503 mRNA_3504 mRNA_3505 mRNA_3506 mRNA_3507
11871 11872 11873 11874 11875
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 -0.2 -0.1 -3.5 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 -0.2 +0.1 -0.5 -0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.0 -0.1 +0.1 -0.5 +0.1
growth on ypd YPD -0.5 +0.1 -0.2 +0.4 -0.1
growth on oleic acid YNB Oleic Acid +0.3 +0.2 -0.2 -0.3 -0.1
growth on oleic acid YNB Glucose +0.4 +0.1 -0.0 -0.3 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.0 +0.2 -0.1 +0.0
facs on lipid accumulation Sorted No BODIPY Gate +0.0 -0.2 +0.1 +0.1 +0.1
auxotrophy Dropout Complete +0.2 +0.2 +0.1 -0.3 +0.0
ypd buoyancy 40 hrs High Buoyancy -0.1 +0.1 +0.3 +0.0 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 +0.3 +0.4 -0.4 -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.0 +0.2 +0.4 -0.4 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 +0.1 +0.3 -0.1 -0.1
growth on ricinoleic acid YNB Glucose +0.1 +0.0 +0.5 -0.3 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 +0.4 -0.3 +0.5 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 +0.3 +0.4 +0.1 +0.2
auxotrophy YNB Glucose +0.3 +0.5 +0.3 -0.1 +0.0
auxotrophy YNB Glucose 75mM Methionine +0.2 +0.4 +0.5 -0.0 +0.0
auxotrophy YNB Glucose 75mM Arginine +0.3 +0.6 +0.4 -0.1 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 +0.0 +0.5 +0.6 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.3 +0.5 +0.8 +0.2
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