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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
11873 and 11874 are separated by 432 nucleotides
11874 and 11875 are separated by 214 nucleotides
11875 and 11876 are separated by 198 nucleotides
11876 and 11877 are separated by 181 nucleotides
mRNA_3505: 11873 - KOG3699 Cytoskeletal protein Adducin, at 257,806 to 259,201
11873
mRNA_3506: 11874 - HMMPfam-Asp/Glu/Hydantoin racemase-PF01177, at 259,634 to 260,419
11874
mRNA_3507: 11875 - K12251 aguB N-carbamoylputrescine amidase, at 260,634 to 261,961
11875
mRNA_3508: 11876 - K00273 DAO, aao D-amino-acid oxidase, at 262,160 to 263,731
11876
mRNA_3509: 11877 - K01464 DPYS, dht, hydA dihydropyrimidinase, at 263,913 to 265,913
11877
Group
Condition
mRNA
_3505
mRNA
_3506
mRNA
_3507
mRNA
_3508
mRNA
_3509
11873
11874
11875
11876
11877
ypd buoyancy
40 hrs No Buoyancy Separation
-0.1
-3.5
-0.2
-0.3
-0.8
facs on lipid accumulation
Sorted Low BODIPY Gate
+0.1
-0.5
-0.2
-0.3
-0.5
growth on oleic acid
YNB Oleic Acid
-0.2
-0.3
-0.1
-0.2
-0.0
growth on oleic acid
YNB Glucose
-0.0
-0.3
-0.1
-0.0
-0.1
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.3
+0.5
+0.2
-0.1
-0.6
auxotrophy
YNB Glucose 75mM Arginine
+0.4
-0.1
+0.0
-0.2
-0.3
facs on lipid accumulation
Sorted High BODIPY Gate
+0.1
-0.5
+0.1
+0.1
+0.1
auxotrophy
Dropout Complete
+0.1
-0.3
+0.0
-0.1
+0.1
ypd buoyancy
40 hrs Low Buoyancy
+0.4
-0.4
+0.1
-0.0
-0.1
auxotrophy
YNB Glucose 75mM Methionine
+0.5
-0.0
+0.0
-0.1
-0.4
growth on ypd
YPD
-0.2
+0.4
-0.1
+0.4
-0.5
auxotrophy
YNB Glucose
+0.3
-0.1
+0.0
-0.1
-0.1
growth on ricinoleic acid
YNB Methylricinoleic Acid
+0.4
-0.4
-0.1
+0.1
+0.0
ypd buoyancy
40 hrs High Buoyancy
+0.3
+0.0
-0.1
+0.1
-0.1
facs on lipid accumulation
Sorted No BODIPY Gate
+0.1
+0.1
+0.1
+0.3
-0.4
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
+0.4
+0.1
+0.2
-0.1
-0.0
growth on ricinoleic acid
YNB Glucose
+0.5
-0.3
+0.2
-0.0
+0.1
ypd buoyancy
40 hrs Median Buoyancy
+0.2
-0.1
+0.0
+0.3
+0.3
growth on ricinoleic acid
YNB Ricinoleic Acid
+0.3
-0.1
-0.1
+0.2
+0.4
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.5
+0.6
+0.3
-0.1
-0.2
buoyancy on lipid accumulation
40 hrs Median Buoyancy
+0.5
+0.8
+0.2
-0.1
-0.2
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