Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11873 and 11874 are separated by 432 nucleotides11874 and 11875 are separated by 214 nucleotides11875 and 11876 are separated by 198 nucleotides11876 and 11877 are separated by 181 nucleotides mRNA_3505: 11873 - KOG3699 Cytoskeletal protein Adducin, at 257,806 to 259,201 11873 mRNA_3506: 11874 - HMMPfam-Asp/Glu/Hydantoin racemase-PF01177, at 259,634 to 260,419 11874 mRNA_3507: 11875 - K12251 aguB N-carbamoylputrescine amidase, at 260,634 to 261,961 11875 mRNA_3508: 11876 - K00273 DAO, aao D-amino-acid oxidase, at 262,160 to 263,731 11876 mRNA_3509: 11877 - K01464 DPYS, dht, hydA dihydropyrimidinase, at 263,913 to 265,913 11877
Group Condition mRNA_3505 mRNA_3506 mRNA_3507 mRNA_3508 mRNA_3509
11873 11874 11875 11876 11877
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 -3.5 -0.2 -0.3 -0.8
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 -0.5 -0.2 -0.3 -0.5
growth on oleic acid YNB Oleic Acid -0.2 -0.3 -0.1 -0.2 -0.0
growth on oleic acid YNB Glucose -0.0 -0.3 -0.1 -0.0 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 +0.5 +0.2 -0.1 -0.6
auxotrophy YNB Glucose 75mM Arginine +0.4 -0.1 +0.0 -0.2 -0.3
facs on lipid accumulation Sorted High BODIPY Gate +0.1 -0.5 +0.1 +0.1 +0.1
auxotrophy Dropout Complete +0.1 -0.3 +0.0 -0.1 +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.4 -0.4 +0.1 -0.0 -0.1
auxotrophy YNB Glucose 75mM Methionine +0.5 -0.0 +0.0 -0.1 -0.4
growth on ypd YPD -0.2 +0.4 -0.1 +0.4 -0.5
auxotrophy YNB Glucose +0.3 -0.1 +0.0 -0.1 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 -0.4 -0.1 +0.1 +0.0
ypd buoyancy 40 hrs High Buoyancy +0.3 +0.0 -0.1 +0.1 -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.1 +0.1 +0.1 +0.3 -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.4 +0.1 +0.2 -0.1 -0.0
growth on ricinoleic acid YNB Glucose +0.5 -0.3 +0.2 -0.0 +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.2 -0.1 +0.0 +0.3 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 -0.1 -0.1 +0.2 +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.5 +0.6 +0.3 -0.1 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.5 +0.8 +0.2 -0.1 -0.2
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