Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11910 and 11911 are separated by 209 nucleotides11911 and 11912 are separated by 1141 nucleotides11912 and 11913 are separated by 194 nucleotides11913 and 11914 are separated by 570 nucleotides mRNA_3542: 11910 - K01597 MVD, mvaD diphosphomevalonate decarboxylase, at 11,016 to 12,700 11910 mRNA_3543: 11911 - HMMPfam-MAGE family-PF01454, at 12,910 to 14,656 11911 mRNA_3544: 11912 - K03506 POLE4 DNA polymerase epsilon subunit 4, at 15,798 to 16,734 11912 mRNA_3545: 11913 - HMMPfam-SAP domain-PF02037,ProSiteProfiles-SAP motif profile.-PS50800,SMART-Putative DNA-binding (bihelical) motif predicted to be involved in chromosomal organisation-SM00513,SUPERFAMILY--SSF68906, at 16,929 to 18,162 11913 mRNA_3546: 11914 - HMMPfam-Domain of unknown function (DUF3844)-PF12955, at 18,733 to 20,553 11914
Group Condition mRNA_3542 mRNA_3543 mRNA_3544 mRNA_3545 mRNA_3546
11910 11911 11912 11913 11914
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -1.3 -0.2 +0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -1.4 +0.1 +0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.2 -0.2 -0.4
growth on oleic acid YNB Glucose N.D. N.D. -0.6 +0.3 -0.3
growth on ypd YPD N.D. N.D. +0.3 -0.6 -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.2 -0.3 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.2 +0.1 -0.3
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.4 -0.1 +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.0 +0.1 -0.4
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.1 -0.4 +0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.2 +0.2 -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.1 +0.0 +0.2
auxotrophy YNB Glucose N.D. N.D. +0.1 -0.0 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.3 -0.3 +0.2
auxotrophy Dropout Complete N.D. N.D. +0.1 +0.2 +0.1
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. +0.4 +0.1 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +0.7 -0.3 +0.0
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +0.9 -0.4 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.8 -0.0 +0.2
growth on oleic acid YNB Oleic Acid N.D. N.D. +0.7 +0.4 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.9 -0.0 +0.2
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