Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11912 and 11913 are separated by 194 nucleotides11913 and 11914 are separated by 570 nucleotides11914 and 11915 are separated by 477 nucleotides11915 and 11916 are separated by 244 nucleotides mRNA_3544: 11912 - K03506 POLE4 DNA polymerase epsilon subunit 4, at 15,798 to 16,734 11912 mRNA_3545: 11913 - HMMPfam-SAP domain-PF02037,ProSiteProfiles-SAP motif profile.-PS50800,SMART-Putative DNA-binding (bihelical) motif predicted to be involved in chromosomal organisation-SM00513,SUPERFAMILY--SSF68906, at 16,929 to 18,162 11913 mRNA_3546: 11914 - HMMPfam-Domain of unknown function (DUF3844)-PF12955, at 18,733 to 20,553 11914 mRNA_3547: 11915 - K02973 RP-S23e, RPS23 small subunit ribosomal protein S23e, at 21,031 to 22,475 11915 mRNA_3548: 11916 - K19307 BMT5 25S rRNA (uracil2634-N3)-methyltransferase, at 22,720 to 24,408 11916
Group Condition mRNA_3544 mRNA_3545 mRNA_3546 mRNA_3547 mRNA_3548
11912 11913 11914 11915 11916
facs on lipid accumulation Sorted Low BODIPY Gate -1.3 -0.2 +0.1 N.D. -0.4
facs on lipid accumulation Sorted High BODIPY Gate -1.4 +0.1 +0.2 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 -0.3 -0.1 N.D. -0.3
ypd buoyancy 40 hrs Low Buoyancy -0.2 -0.2 -0.4 N.D. +0.3
growth on ypd YPD +0.3 -0.6 -0.3 N.D. +0.3
auxotrophy YNB Glucose +0.1 -0.0 +0.1 N.D. -0.3
growth on oleic acid YNB Glucose -0.6 +0.3 -0.3 N.D. +0.5
growth on ricinoleic acid YNB Glucose +0.1 -0.4 +0.1 N.D. +0.1
ypd buoyancy 40 hrs High Buoyancy +0.0 +0.1 -0.4 N.D. +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 -0.3 +0.2 N.D. -0.2
ypd buoyancy 40 hrs Median Buoyancy -0.2 +0.2 -0.1 N.D. +0.2
auxotrophy YNB Glucose 75mM Methionine -0.1 +0.0 +0.2 N.D. -0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.4 -0.1 +0.2 N.D. +0.4
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 +0.1 -0.3 N.D. +0.6
auxotrophy YNB Glucose 75mM Arginine +0.4 +0.1 -0.0 N.D. +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.7 -0.3 +0.0 N.D. +0.1
auxotrophy Dropout Complete +0.1 +0.2 +0.1 N.D. +0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.9 -0.4 +0.2 N.D. +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.8 -0.0 +0.2 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.9 -0.0 +0.2 N.D. +0.2
growth on oleic acid YNB Oleic Acid +0.7 +0.4 +0.1 N.D. +0.3
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