Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 11942 and 11943 are separated by 670 nucleotides11943 and 11944 are separated by 468 nucleotides11944 and 11945 are separated by 997 nucleotides11945 and 11946 are separated by 370 nucleotides mRNA_3574: 11942 - ProSiteProfiles-Eukaryotic RNA Recognition Motif (RRM) profile.-PS50102,SMART-RNA recognition motif-SM00360,SUPERFAMILY--SSF54928, at 84,191 to 85,564 11942 mRNA_3575: 11943 - Hypothetical Protein, at 86,235 to 86,923 11943 mRNA_3576: 11944 - Hypothetical Protein, at 87,392 to 87,994 11944 mRNA_3577: 11945 - K04567 KARS, lysS lysyl-tRNA synthetase, class II, at 88,992 to 92,589 11945 mRNA_3578: 11946 - HMMPfam-Glutathione-dependent formaldehyde-activating enzyme-PF04828,SUPERFAMILY--SSF51316, at 92,960 to 93,719 11946
Group Condition mRNA_3574 mRNA_3575 mRNA_3576 mRNA_3577 mRNA_3578
11942 11943 11944 11945 11946
growth on oleic acid YNB Glucose +0.1 -0.7 N.D. -0.5 N.D.
growth on oleic acid YNB Oleic Acid -0.0 -0.4 N.D. -0.4 N.D.
auxotrophy YNB Glucose +0.0 -0.5 N.D. -0.1 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 +0.0 N.D. -0.9 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.1 -0.2 N.D. -0.5 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 -0.1 N.D. -0.1 N.D.
auxotrophy Dropout Complete -0.4 -0.4 N.D. +0.3 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.3 -0.1 N.D. -0.4 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 -0.1 N.D. +0.1 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.0 -0.3 N.D. +0.1 N.D.
growth on ricinoleic acid YNB Glucose -0.4 +0.2 N.D. +0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.4 -0.2 N.D. -0.2 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.2 +0.2 N.D. -0.2 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.2 -0.6 N.D. +0.5 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.1 +0.2 N.D. +0.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 +0.6 N.D. -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 +0.5 N.D. +0.2 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.4 +0.7 N.D. +0.3 N.D.
growth on ypd YPD +0.5 -0.5 N.D. +0.6 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 +0.5 N.D. +0.5 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.6 +0.6 N.D. -0.0 N.D.
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